Rabbit anti-Human, Mouse MAPK1 Polyclonal Antibody | anti-MAPK1 antibody

MAPK1 Antibody

Cat. Num. AAA231813

• Gene Names: MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: MAPK1, Antibody; MAPK1 Antibody; Mitogen-activated protein kinase 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase; MAPK1; ERK2; PRKM1; PRKM2; anti-MAPK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 360

• Applicable Applications for anti-MAPK1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Species: Human
• Immunogen: Recombinant human Mitogen-activated protein kinase 1 protein
• Conjugate: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-154 / sc-153
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescent analysis of HepG-2 cells using AAA231813 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemisry)

(IHC image of AAA231813 diluted at 1:600 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohiostchemistry)

(IHC image of AAA231813 diluted at 1:600 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western BlotPositive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate,293 whole cell lysate,Rat lung tissueAll lanes: MAPK1 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42,37 KDaObserved band size: 42,37 KDa)

Related Product Information for anti-MAPK1 antibody

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 66932916
• NCBI GeneID: 5594
• NCBI Accession #: NP_002736.3
• NCBI GenBank Nucleotide #: NM_002745.4
• UniProt Accession #: P28482; A8CZ64
• Molecular Weight: 36,432 Da
• NCBI Official Full Name: mitogen-activated protein kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 1
• NCBI Official Symbol: MAPK1
• NCBI Official Synonym Symbols: ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase 1
• UniProt Gene Name: MAPK1
• UniProt Synonym Gene Names: ERK2; PRKM1; PRKM2; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2
• UniProt Entry Name: MK01_HUMAN