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IF (Immunofluorescence)
(Immunofluorescence staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-HUMAN MAPK11 polyclonal antibody (dilution ratio: 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Rabbit MAPK11 Polyclonal Antibody | anti-MAPK11 antibody
Anti-MAPK11 Antibody, Rabbit Polyclonal
Gene Names
MAPK11; P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MAPK11, Antibody; Anti-MAPK11 Antibody, Rabbit Polyclonal; MAPK11 Antibody, Rabbit PAb, Antigen Affinity Purified; mitogen-activated protein kinase 11; Anti-p38-2 Antibody; Anti-P38B Antibody; Anti-p38Beta Antibody; Anti-P38BETA2 Antibody; Anti-PRKM11 Antibody; Anti-SAPK2 Antibody; Anti-SAPK2B Antibody; anti-MAPK11 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MAPK11
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MAPK11 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human MAPK11
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MAPK11, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-HUMAN MAPK11 polyclonal antibody (dilution ratio: 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-HUMAN MAPK11 polyclonal antibody (dilution ratio: 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IHC (Immunohistochemisry)
(Immunochemical staining of human MAPK11 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human MAPK11 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human MAPK11 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human MAPK11 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-MAPK11 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:36 kDa)
WB (Western Blot)
(Anti-MAPK11 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:36 kDa)
Related Product Information for anti-MAPK11 antibody
The p38 kinases are one of the four subgroups of the mitogen-activated protein kinase (MAPK) superfamily which are involved in innate immunity. The p38 subfamily includes four members namely p38alpha (MAPK14), p38beta (MAPK11), p38gamma (MAPK12), and p38delta (MAPK13), regulates the activation of several transcription factors. MAPK11 (Mitogen-Activated Protein Kinase 11) is a Protein Coding gene. This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. MAPK11 is widely expressed in fat, the brain, and other tissues. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen-activated protein kinase kinases (MKKs).
Product Categories/Family for anti-MAPK11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
364
NCBI Official Full Name
Mitogen-activated protein kinase 11
NCBI Official Synonym Full Names
mitogen-activated protein kinase 11
NCBI Official Symbol
MAPK11
NCBI Official Synonym Symbols
P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
NCBI Protein Information
mitogen-activated protein kinase 11; MAP kinase 11; MAP kinase p38 beta; stress-activated protein kinase-2; stress-activated protein kinase-2b; mitogen-activated protein kinase p38-2; mitogen-activated protein kinase p38 beta
UniProt Protein Name
Mitogen-activated protein kinase 11
UniProt Gene Name
MAPK11
UniProt Synonym Gene Names
PRKM11; SAPK2; SAPK2B; MAP kinase 11; MAPK 11; MAP kinase p38 beta; p38b; SAPK2b
UniProt Entry Name
MK11_HUMAN
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Product Notes
The MAPK11 mapk11 (Catalog #AAA254407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MAPK11 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK11 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MAPK11 mapk11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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