IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit MAPK7 Polyclonal Antibody | anti-MAPK7 antibody
MAPK7 Antibody (C-term)
Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MAPK7, Antibody; MAPK7 Antibody (C-term); Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Big MAP kinase 1; BMK-1; Extracellular signal-regulated kinase 5; ERK-5; MAPK7; BMK1; ERK5; PRKM7; anti-MAPK7 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MAPK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 776-805 amino acids from the C-terminal region of human MAPK7.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
776-805
Sequence Length
451
Applicable Applications for anti-MAPK7 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Plays a role in various cellular processes suchas proliferation, differentiation and cellsurvival. The upstream activator of MAPK7 isthe MAPK kinase MAP2K5. Upon activation, ittranslocates to the nucleus andphosphorylates various downstream targetsincluding MEF2C. EGF activates MAPK7through a Ras- independent andMAP2K5-dependent pathway. May have a rolein muscle cell differentiation. May beimportant for endothelial function andmaintenance of blood vessel integrity.MAP2K5 and MAPK7 interact specifically withone another and not with MEK1/ERK1 orMEK2/ERK2 pathways. Phosphorylates SGK1at Ser-78 and this is required for growthfactor-induced cell cycle progression.Involved in the regulation of p53/TP53 bydisrupting the PML-MDM2 interaction.
Cellular location
Cytoplasm. Nucleus. Nucleus, PML body.Note=Translocates to the nucleus uponactivation
Tissue location
Expressed in many adult tissues. Abundant inheart, placenta, lung, kidney and skeletalmuscle. Not detectable in liver.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of anti-ERK5 C-term Pab in K562 cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-ERK5 C-term Pab in K562 cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.)
Related Product Information for anti-MAPK7 antibody
MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
Product Categories/Family for anti-MAPK7 antibody
References
Zhou, G., et al., J. Biol. Chem. 270(21):12665-12669 (1995).
Lee, J.D., et al., Biochem. Biophys. Res. Commun. 213(2):715-724 (1995).
Lee, J.D., et al., Biochem. Biophys. Res. Commun. 213(2):715-724 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88386
NCBI Official Full Name
mitogen-activated protein kinase 7 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 7
NCBI Official Symbol
MAPK7
NCBI Official Synonym Symbols
BMK1; ERK4; ERK5; PRKM7
NCBI Protein Information
mitogen-activated protein kinase 7
UniProt Protein Name
Mitogen-activated protein kinase 7
UniProt Gene Name
MAPK7
UniProt Synonym Gene Names
BMK1; ERK5; PRKM7; MAP kinase 7; MAPK 7; BMK-1; ERK-5
UniProt Entry Name
MK07_HUMAN
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Product Notes
The MAPK7 mapk7 (Catalog #AAA289435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 776-805. The MAPK7 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAPK7 mapk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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