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WB (Western Blot)
(WB Suggested Anti-MAPT Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
Rabbit MAPT Polyclonal Antibody | anti-MAPT antibody
MAPT antibody - middle region
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Pig, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
MAPT, Antibody; MAPT antibody - middle region; anti-MAPT antibody
Host
Rabbit
Reactivity
Human, Mouse, Pig, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: RGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPG
Sequence Length
441
Applicable Applications for anti-MAPT antibody
WB (Western Blot), IHC (Immunohistochemistry)
Homology
Human: 100%; Mouse: 86%; Pig: 79%; Rat: 86%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human MAPT
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-MAPT Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
WB (Western Blot)
(WB Suggested Anti-MAPT Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
WB (Western Blot)
(WB Suggested Anti-MAPT AntibodyPositive Control: Lane 1: 10ug N2A expressed human Tau-C Lane 2: 10ug N2A expressed human Tau-E Lane 3: 10ug N2A expressed human Tau-F Lane 4: 10ug N2A expressed human Tau-B Lane 5: 10ug N2A expressed human Fetal-Tau Lane 6: 10ug N2A expressed human Tau-D Lane 7: 10ug N2A expressed human much Fetal-Tau Lane 8: 10ug N2A lysatePrimary Antibody Dilution : 1:2000Secondary Antibody : Donkey anti-rabbit-HRPSecondry Antibody Dilution : 1:10,000Submitted by: Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig)
WB (Western Blot)
(WB Suggested Anti-MAPT AntibodyPositive Control: Lane 1: 10ug N2A expressed human Tau-C Lane 2: 10ug N2A expressed human Tau-E Lane 3: 10ug N2A expressed human Tau-F Lane 4: 10ug N2A expressed human Tau-B Lane 5: 10ug N2A expressed human Fetal-Tau Lane 6: 10ug N2A expressed human Tau-D Lane 7: 10ug N2A expressed human much Fetal-Tau Lane 8: 10ug N2A lysatePrimary Antibody Dilution : 1:2000Secondary Antibody : Donkey anti-rabbit-HRPSecondry Antibody Dilution : 1:10,000Submitted by: Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig)
WB (Western Blot)
(Researcher: Max Holzer, Paul Flechsig Institut of Brain Research, University of LeipzigApplication: Western blottingSpecies + Tissue/Cell type:Lane 1: 10ug E.Coli: expressed human Tau-CLane 2: 10ug E.Coli: expressed human Tau-ELane 3: 10ug E.Coli: expressed human Tau-FLane 4: 10ug E.Coli: expressed human Tau-BLane 5: 10ug E.Coli: expressed human Fetal-TauLane 6: 10ug E.Coli: expressed human Tau-DPrimary antibody dilution: 1:1000Secondary antibody: Donkey anti-rabbit-HRPSecondary antibody dilution: 1:10,000)
WB (Western Blot)
(Researcher: Max Holzer, Paul Flechsig Institut of Brain Research, University of LeipzigApplication: Western blottingSpecies + Tissue/Cell type:Lane 1: 10ug E.Coli: expressed human Tau-CLane 2: 10ug E.Coli: expressed human Tau-ELane 3: 10ug E.Coli: expressed human Tau-FLane 4: 10ug E.Coli: expressed human Tau-BLane 5: 10ug E.Coli: expressed human Fetal-TauLane 6: 10ug E.Coli: expressed human Tau-DPrimary antibody dilution: 1:1000Secondary antibody: Donkey anti-rabbit-HRPSecondary antibody dilution: 1:10,000)
IHC (Immunohistochemistry)
(Sample Type :A: Transgenic hTau P301L mouse cortexB: Tau-Ko mouse cortexC: WT mouse cortex Primary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-biotin / Avidin-HRP Secondary Antibody Dilution :1:1000 /1:2000 Color/Signal Descriptions :Dark grey: MAPT Gene Name :MAPTSubmitted by :Max Holzer,Paul Flechsig Institut of Brain Research,University of Leipzig)
IHC (Immunohistochemistry)
(Sample Type :A: Transgenic hTau P301L mouse cortexB: Tau-Ko mouse cortexC: WT mouse cortex Primary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-biotin / Avidin-HRP Secondary Antibody Dilution :1:1000 /1:2000 Color/Signal Descriptions :Dark grey: MAPT Gene Name :MAPTSubmitted by :Max Holzer,Paul Flechsig Institut of Brain Research,University of Leipzig)
Related Product Information for anti-MAPT antibody
This is a rabbit polyclonal antibody against MAPT. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: MAPT is differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. The mutations in the gene have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Target Description: MAPT is differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. The mutations in the gene have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Product Categories/Family for anti-MAPT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46kDa
NCBI Official Full Name
microtubule-associated protein tau isoform 2
NCBI Official Synonym Full Names
microtubule associated protein tau
NCBI Official Symbol
MAPT
NCBI Official Synonym Symbols
TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
NCBI Protein Information
microtubule-associated protein tau
UniProt Protein Name
Microtubule-associated protein tau
UniProt Gene Name
MAPT
UniProt Synonym Gene Names
MAPTL; MTBT1; TAU; PHF-tau
UniProt Entry Name
TAU_HUMAN
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Product Notes
The MAPT mapt (Catalog #AAA199859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAPT antibody - middle region reacts with Human, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAPT can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the MAPT mapt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: RGAAPPGQKG QANATRIPAK TPPAPKTPPS SGEPPKSGDR SGYSSPGSPG. It is sometimes possible for the material contained within the vial of "MAPT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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