Rabbit MARCH 8 Polyclonal Antibody | anti-MARCH8 antibody

MARCH8 Antibody

Cat. Num. AAA39606

• Gene Names: MARCH8; MIR; CMIR; c-MIR; RNF178; MARCH-VIII
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Western Blot, ELISA
• Purity: MARCH8 Antibody is affinity chromatography purified via peptide column.
• Synonyms: MARCH 8, Antibody; MARCH8 Antibody; MARCH8; MIR; c-MIR; RNF178; MARCH-VIII; E3 ubiquitin-protein ligase MARCH8; Cellular modulator of immune recognition; membrane-associated ring finger (C3HC4) 8; anti-MARCH8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: MARCH8 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 291

• Applicable Applications for anti-MARCH8 antibody: ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8.
• Buffer: MARCH8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: MARCH8 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ICC (Immunocytochemistry)

(Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.)

Related Product Information for anti-MARCH8 antibody

MARCH8 Antibody: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 50539410
• NCBI GeneID: 220972
• NCBI Accession #: NP_659458
• NCBI GenBank Nucleotide #: NM_145021.5
• UniProt Accession #: Q5T0T0; Q5T0S8; Q8TC72; B2R8E7; H0Y7C6
• Molecular Weight: 63,319 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH8 isoform a
• NCBI Official Synonym Full Names: membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
• NCBI Official Symbol: MARCH8
• NCBI Official Synonym Symbols: MIR; CMIR; c-MIR; RNF178; MARCH-VIII
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH8; RING finger protein 178; membrane-associated RING-CH protein VIII; membrane-associated RING finger protein 8; c-mir, cellular modulator of immune recognition; cellular modulator of immune recognition (c-MIR)
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH8
• UniProt Gene Name: MARCH8
• UniProt Synonym Gene Names: MIR; RNF178; c-MIR; MARCH-VIII
• UniProt Entry Name: MARH8_HUMAN