IF (Immunofluorescence)
(Figure 2. IF analysis of MARCHF2 using anti-MARCHF2 antibody (AAA127793).MARCHF2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MARCHF2 Antibody (AAA127793) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit anti-Human MARCHF2 Polyclonal Antibody | anti-MARCHF2 antibody
Anti-MARCHF2 Antibody Picoband
Gene Names
MARCH2; RNF172; HSPC240; MARCH-II
Reactivity
Human
Applications
ELISA, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
MARCHF2, Antibody; Anti-MARCHF2 Antibody Picoband; Follicular dendritic cell secreted peptide; RNA-binding protein 47; RNA-binding motif protein 47; RBM47; anti-MARCHF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-MARCHF2 antibody
ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E coli-derived human MARCHF2 recombinant protein (Position: M1-V246).
Subcellular Localization
Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins
Protein Function
Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of MARCHF2 using anti-MARCHF2 antibody (AAA127793).MARCHF2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MARCHF2 Antibody (AAA127793) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of MARCHF2 using anti-MARCHF2 antibody (AAA127793).MARCHF2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MARCHF2 Antibody (AAA127793) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of MARCHF2 using anti-MARCHF2 antibody (AAA127793).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCHF2 antigen affinity purified polyclonal antibody (#AAA127793) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MARCHF2 at approximately 27 kDa. The expected band size for MARCHF2 is at 27 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of MARCHF2 using anti-MARCHF2 antibody (AAA127793).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCHF2 antigen affinity purified polyclonal antibody (#AAA127793) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MARCHF2 at approximately 27 kDa. The expected band size for MARCHF2 is at 27 kDa.)
Related Product Information for anti-MARCHF2 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-MARCHF2 antibody
References
1. Bartee, E., Mansouri, M., Nerenberg, B. T. H., Gouveia, K., Fruh, K. Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins. J. Virology 78: 1109-1120, 2004. 2. De Gassart, A., Camosseto, V., Thibodeau, J., Ceppi, M., Catalan, N., Pierre, P., Gatti, E. MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc. Nat. Acad. Sci. 105: 3491-3496, 2008.3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/29/2010.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,995 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH2 isoform 1
NCBI Official Synonym Full Names
membrane-associated ring finger (C3HC4) 2, E3 ubiquitin protein ligase
NCBI Official Symbol
MARCH2
NCBI Official Synonym Symbols
RNF172; HSPC240; MARCH-II
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH2; RING finger protein 172; membrane-associated RING-CH protein II; membrane-associated RING finger protein 2
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH2
UniProt Gene Name
MARCH2
UniProt Synonym Gene Names
RNF172; MARCH-II
UniProt Entry Name
MARH2_HUMAN
Similar Products
Product Notes
The MARCHF2 march2 (Catalog #AAA127793) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MARCHF2 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MARCHF2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MARCHF2 march2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCHF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.