FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-MARCO antibody (AAA128237).Overlay histogram showing THP-1 cells stained with AAA128237 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MARCO Antibody (AAA128237, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit anti-Human MARCO Polyclonal Antibody | anti-MARCO antibody
Anti-MARCO Antibody Picoband
Gene Names
MARCO; SR-A6; SCARA2
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
MARCO, Antibody; Anti-MARCO Antibody Picoband; Macrophage receptor MARCO; MARCO; SCARA2; Macrophage receptor with collagenous structure; Scavenger receptor class A member 2; anti-MARCO antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-MARCO antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human MARCO recombinant protein (Position: M1-R489). Human MARCO shares 66.5% amino acid (aa) sequence identity with mouse MARCO.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Cross Reactivity
No cross-reactivity with other proteins
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-MARCO antibody (AAA128237).Overlay histogram showing THP-1 cells stained with AAA128237 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MARCO Antibody (AAA128237, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-MARCO antibody (AAA128237).Overlay histogram showing THP-1 cells stained with AAA128237 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MARCO Antibody (AAA128237, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of MARCO using anti-MARCO antibody (AAA128237).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U2OS whole cell lysates,Lane 2: human Hacat whole cell lysates,Lane 3: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCO antigen affinity purified polyclonal antibody (AAA128237) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MARCO at approximately 53 kDa. The expected band size for MARCO is at 53 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of MARCO using anti-MARCO antibody (AAA128237).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U2OS whole cell lysates,Lane 2: human Hacat whole cell lysates,Lane 3: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCO antigen affinity purified polyclonal antibody (AAA128237) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MARCO at approximately 53 kDa. The expected band size for MARCO is at 53 kDa.)
Related Product Information for anti-MARCO antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-MARCO antibody
References
1. Arredouani, M., Yang, Z., Ning, Y. Y., Qin, G., Soininen, R., Tryggvason, K., Kobzik, L. The scavenger receptor MARCO is required for lung defense against Pneumococcal pneumonia and inhaled particles. J. Exp. Med. 200: 267-272, 2004.2. Dorrington, M. G., Roche, A. M., Chauvin, S. E., Tu, Z., Mossman, K. L., Weiser, J. N., Bowdish, D. M. E. MARCO is required for TLR2- and Nod2-mediated responses to Streptococcus pneumoniae and clearance of pneumococcal colonization in the murine nasopharynx. J. Immun. 190: 250-258, 2013.3. Elomaa, O., Kangas, M., Sahlberg, C., Tuukkanen, J., Sormunen, R., Liakka, A., Thesleff, I., Kraal, G., Tryggvason, K. Cloning of a novel bacteria-binding receptor structurally related to scavenger receptors and expressed in a subset of macrophages. Cell 80: 603-609, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,865 Da
NCBI Official Full Name
macrophage receptor MARCO
NCBI Official Synonym Full Names
macrophage receptor with collagenous structure
NCBI Official Symbol
MARCO
NCBI Official Synonym Symbols
SR-A6; SCARA2
NCBI Protein Information
macrophage receptor MARCO
UniProt Protein Name
Macrophage receptor MARCO
UniProt Gene Name
MARCO
UniProt Synonym Gene Names
SCARA2
Similar Products
Product Notes
The MARCO marco (Catalog #AAA128237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MARCO Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MARCO can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the MARCO marco for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.