WB (Western Blot)
(Detection of human MASTL by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody AAA211797 (lot AAA211797-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human MASTL Polyclonal Antibody | anti-MASTL antibody
Rabbit anti-MASTL Antibody, Affinity Purified
Gene Names
Mastl; GW; GWL; THC2; C88295; MAST-L; 2700091H24Rik
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MASTL, Antibody; Rabbit anti-MASTL Antibody, Affinity Purified; GW; THC2; serine/threonine-protein kinase greatwall; Microtubule-associated serine/threonine-protein kinase-like; MAST-L; hGWL; greatwall protein kinase; greatwall kinase homolog; GREATWALL; GWL; Serine/threonine-protein kinase greatwall; microtubule associated serine/threonine kinase like; anti-MASTL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MASTL antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 575 and 625
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MASTL by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody AAA211797 (lot AAA211797-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human MASTL by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody AAA211797 (lot AAA211797-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MASTL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MASTL antibody AAA211797 (lot AAA211797-4) used for IP at 3 ug per reaction. MASTL was also immunoprecipitated by a previous lot of this antibody (lot AAA211797-3). For blotting immunoprecipitated MASTL, AAA211797 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MASTL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MASTL antibody AAA211797 (lot AAA211797-4) used for IP at 3 ug per reaction. MASTL was also immunoprecipitated by a previous lot of this antibody (lot AAA211797-3). For blotting immunoprecipitated MASTL, AAA211797 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human MASTL by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MASTL (Cat. No. AAA211797 Lot4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MASTL by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MASTL (Cat. No. AAA211797 Lot4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-MASTL antibody
MASTL (microtubule-associated serine/threonine-protein kinase-like) is an uncharacterized putative serine/threonine kinase which may be involved in megakaryocyte development. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) a disease characterized by decreased circulating platelets and increased potential for bleeding.
Product Categories/Family for anti-MASTL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95,975 Da
NCBI Official Full Name
serine/threonine-protein kinase greatwall
NCBI Official Synonym Full Names
microtubule associated serine/threonine kinase-like
NCBI Official Symbol
Mastl
NCBI Official Synonym Symbols
GW; GWL; THC2; C88295; MAST-L; 2700091H24Rik
NCBI Protein Information
serine/threonine-protein kinase greatwall; microtubule-associated serine/threonine-protein kinase-like
UniProt Protein Name
Serine/threonine-protein kinase greatwall
UniProt Gene Name
MASTL
UniProt Synonym Gene Names
GW; GWL; THC2; GW; GWL; hGWL; MAST-L
UniProt Entry Name
GWL_HUMAN
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Product Notes
The MASTL mastl (Catalog #AAA211797) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MASTL Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MASTL can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MASTL mastl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MASTL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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