WB (Western Blot)
(Detection of human Matriptase/ST14 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, Hep-G2, RPMI-8226, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Matriptase/ST14 antibody (AAA210825 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Matriptase/ST14 Polyclonal Antibody | anti-ST14 antibody
Rabbit anti-Matriptase/ST14 Antibody, Affinity Purified
Gene Names
ST14; HAI; MTSP1; SNC19; MT-SP1; PRSS14; TADG15; TMPRSS14
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Matriptase/ST14, Antibody; Rabbit anti-Matriptase/ST14 Antibody, Affinity Purified; Tumor-associated differentially-expressed gene 15 protein; tumor associated differentially expressed gene 15 protein; TMPRSS14; serine protease TADG-15; suppressor of tumorigenicity 14 protein; suppression of tumorigenicity 14 (colon carcinoma); SNC19; suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); HAI; TADG15; ARCI11; Matriptase; membrane-type serine protease 1; MTSP1; serine protease 14; MT-SP1; prostamin; PRSS14; Suppressor of tumorigenicity 14 protein; suppression of tumorigenicity 14; anti-ST14 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ST14 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 805 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Matriptase/ST14 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, Hep-G2, RPMI-8226, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Matriptase/ST14 antibody (AAA210825 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Matriptase/ST14 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, Hep-G2, RPMI-8226, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Matriptase/ST14 antibody (AAA210825 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Matriptase/ST14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from RPMI-8226 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Matriptase/ST14 antibody (AAA210825 lot 3) used for IP at 6 ug per reaction. Matriptase/ST14 was also immunoprecipitated by a previous lot of this antibody (AAA210825 lot 2) and a second antibody against a different epitope of Matriptase/ST14 (BL10867). For blotting immunoprecipitated Matriptase/ST14, AAA210825 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human Matriptase/ST14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from RPMI-8226 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Matriptase/ST14 antibody (AAA210825 lot 3) used for IP at 6 ug per reaction. Matriptase/ST14 was also immunoprecipitated by a previous lot of this antibody (AAA210825 lot 2) and a second antibody against a different epitope of Matriptase/ST14 (BL10867). For blotting immunoprecipitated Matriptase/ST14, AAA210825 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-ST14 antibody
Matriptase/ST14 is an epithelial-derived type II transmembrane trypsin-like serine protease that has been shown to cleave and activate various proteases and growth factors. The overexpression of Matriptase /ST14 in a variety of tumors, and its ability to degrade extracellular matrix suggests a role in tumor invasion.
Product Categories/Family for anti-ST14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94,770 Da
NCBI Official Full Name
suppressor of tumorigenicity 14 protein
NCBI Official Synonym Full Names
suppression of tumorigenicity 14 (colon carcinoma)
NCBI Official Symbol
ST14
NCBI Official Synonym Symbols
HAI; MTSP1; SNC19; MT-SP1; PRSS14; TADG15; TMPRSS14
NCBI Protein Information
suppressor of tumorigenicity 14 protein; prostamin; serine protease 14; serine protease TADG-15; membrane-type serine protease 1; tumor associated differentially expressed gene 15 protein; tumor-associated differentially-expressed gene 15 protein; suppres
UniProt Protein Name
Suppressor of tumorigenicity 14 protein
UniProt Gene Name
ST14
UniProt Synonym Gene Names
PRSS14; SNC19; TADG15; MT-SP1
UniProt Entry Name
ST14_HUMAN
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Product Notes
The ST14 st14 (Catalog #AAA210825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Matriptase/ST14 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Matriptase/ST14 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ST14 st14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Matriptase/ST14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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