Rabbit anti-Human MCAF2 Polyclonal Antibody | anti-ATF7IP2 antibody

MCAF2 Antibody (Center)

Cat. Num. AAA287823

• Gene Names: ATF7IP2; MCAF2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MCAF2, Antibody; MCAF2 Antibody (Center); Activating transcription factor 7-interacting protein 2; ATF7-interacting protein 2; MBD1-containing chromatin-associated factor 2; ATF7IP2; MCAF2; anti-ATF7IP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This MCAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-347 amino acids from the Central region of human MCAF2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 318-347
• Sequence Length: 543

• Applicable Applications for anti-ATF7IP2 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Cellular Location: Nucleus
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(MCAF2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(MCAF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCAF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(MCAF2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).)

Related Product Information for anti-ATF7IP2 antibody

Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone 'Lys-9' trimethylation activity (Probable).

References

Adkins, D.E., et al. Mol. Psychiatry (2010) In press :
Ichimura, T., et al. J. Biol. Chem. 280(14):13928-13935(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 371872694
• NCBI GeneID: 80063
• NCBI Accession #: NP_001243089.1; NP_079273.2
• NCBI GenBank Nucleotide #: NM_001256160.1
• UniProt Accession #: Q5U623; Q53EZ7; Q658U2; Q6IS97; Q8N9X8; Q9H9L6; B2RNR2
• NCBI Official Full Name: activating transcription factor 7-interacting protein 2 isoform 2
• NCBI Official Synonym Full Names: activating transcription factor 7 interacting protein 2
• NCBI Official Symbol: ATF7IP2
• NCBI Official Synonym Symbols: MCAF2
• NCBI Protein Information: activating transcription factor 7-interacting protein 2
• UniProt Protein Name: Activating transcription factor 7-interacting protein 2
• UniProt Gene Name: ATF7IP2
• UniProt Synonym Gene Names: MCAF2; ATF7-interacting protein 2
• UniProt Entry Name: MCAF2_HUMAN