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IEM (Immunogold Electron Microscopy)
(Immunogold labeling of RPE, in vivo injected with mCherry expressing vector)
Goat mCherry Polyclonal Antibody | anti-mCherry antibody
mCherry Polyclonal Antibody
Reactivity
Transfected cells proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry
Synonyms
mCherry, Antibody; mCherry Polyclonal Antibody; Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody; anti-mCherry antibody
Host
Goat
Reactivity
Transfected cells proteins
Clonality
Polyclonal
Isotype
IgG
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody does not recognize GFP (green flourescent protein).
Form/Format
Polyclonal antibody supplied as a 200ul aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Sequence Length
236
Applicable Applications for anti-mCherry antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunogold Electron Microscopy (IEM)
Application Notes
WB:1:500-1:5,000
IF: 1:50-1:500
IHC(P): 1:50-1:500
IHC(F): 1:50-1:500
IEM: 1:50-1:500
IF: 1:50-1:500
IHC(P): 1:50-1:500
IHC(F): 1:50-1:500
IEM: 1:50-1:500
Immunogen
Purified recombinant peptide produced in E. coli.
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
Store at -20°C for long term storage. Store at 2-8°C short term. Avoid freeze/thaw cycles.
IEM (Immunogold Electron Microscopy)
(Immunogold labeling of RPE, in vivo injected with mCherry expressing vector)
IEM (Immunogold Electron Microscopy)
(Immunogold labeling of RPE, in vivo injected with mCherry expressing vector)
IF (Immunofluorescence)
(Immunofluorescence – anti-mCherry Ab using hCEC cells transduced with mCherry-Rab11a; cells were fixed with methanol and anti-Ra11a at 1/250)
IF (Immunofluorescence)
(Immunofluorescence – anti-mCherry Ab using hCEC cells transduced with mCherry-Rab11a; cells were fixed with methanol and anti-Ra11a at 1/250)
IF (Immunofluorescence)
(Anti-mCherry Ab in COS-7 cells transfected with mCherry-EEA1 at 1/100 dilution)
IF (Immunofluorescence)
(Anti-mCherry Ab in COS-7 cells transfected with mCherry-EEA1 at 1/100 dilution)
IF (Immunofluorescence)
(293 HEK cells transduced with Ad mCherry Rab1a and stained with anti-mCherry)
IF (Immunofluorescence)
(293 HEK cells transduced with Ad mCherry Rab1a and stained with anti-mCherry)
IF (Immunofluorescence)
(Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing mCherry in neurons (DyGlutmcherry) using 1st Ab anti-mCherry at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight®488 at 1/500)
IF (Immunofluorescence)
(Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing mCherry in neurons (DyGlutmcherry) using 1st Ab anti-mCherry at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight®488 at 1/500)
IF (Immunofluorescence)
(Immunofluorescence – anti-mCherry Ab using MCR cells transduced with mCherry-Rab11a; cells were fixed with methanol and anti-Rab11a at 1/250)
IF (Immunofluorescence)
(Immunofluorescence – anti-mCherry Ab using MCR cells transduced with mCherry-Rab11a; cells were fixed with methanol and anti-Rab11a at 1/250)
Application Data
(Anti-mCherry Ab at 1/1,000 dilution;293 cells transfected with myc-mCherry)
Application Data
(Anti-mCherry Ab at 1/1,000 dilution;293 cells transfected with myc-mCherry)
Reactivity Chart
Reactivity Chart
Related Product Information for anti-mCherry antibody
Goat polyclonal antibody to mCherry (Cherry fluorescent protein).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
mCherry, partial
Similar Products
Product Notes
The mCherry (Catalog #AAA13867) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The mCherry Polyclonal Antibody reacts with Transfected cells proteins and may cross-react with other species as described in the data sheet. AAA Biotech's mCherry can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunogold Electron Microscopy (IEM). WB:1:500-1:5,000 IF: 1:50-1:500 IHC(P): 1:50-1:500 IHC(F): 1:50-1:500 IEM: 1:50-1:500. Researchers should empirically determine the suitability of the mCherry for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mCherry, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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