WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using MCM10 antibody.)
Rabbit MCM10 Polyclonal Antibody | anti-MCM10 antibody
MCM10 Antibody
Gene Names
MCM10; CNA43; DNA43; PRO2249
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MCM10, Antibody; MCM10 Antibody; CNA43; DNA43; FLJ31133; Homolog of yeast MCM10; HsMCM10; mcm10; MCM10 minichromosome maintenance deficient 10; MCM10_HUMAN; Minichromosome maintenance 10 replication initiation factor; Minichromosome maintenance complex component 10; Minichromosome maintenance protein 10 isoform 1; PRO2249; Protein MCM10 homolog; anti-MCM10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MCM10 antibody detects endogenous levels of MCM10.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
874
Applicable Applications for anti-MCM10 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human MCM10.
Subcellular Location
Nucleus
Tissue Specificity
Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G1 phase, and starts to accumulate at the onset of S phase.
Predicted Cross Reactivity
Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Zebrafish (92%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (92%), Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using MCM10 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using MCM10 antibody.)
IHC (Immunohiostchemistry)
(AAA322986 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA322986 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using MCM10 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using MCM10 antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-MCM10 antibody
Function: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (PubMed:24726359).
Subunit Structure: Self-associates (By similarity). Interacts with ORC2. May interact with MCM2 and MCM6. Interacts with the DNA polymerase alpha subunit POLA1. Interacts with RECQL4; this interaction regulates RECQL4 unwinding activity. Interacts with WDHD1.
Similarity: Each zinc finger-like domain binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain.The N-terminal domain mediates homodimerization. Belongs to the MCM10 family.
Subunit Structure: Self-associates (By similarity). Interacts with ORC2. May interact with MCM2 and MCM6. Interacts with the DNA polymerase alpha subunit POLA1. Interacts with RECQL4; this interaction regulates RECQL4 unwinding activity. Interacts with WDHD1.
Similarity: Each zinc finger-like domain binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain.The N-terminal domain mediates homodimerization. Belongs to the MCM10 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 97-105 kDa
Predicted: 99 kDa
Predicted: 99 kDa
NCBI Official Full Name
protein MCM10 homolog isoform 2
NCBI Official Synonym Full Names
minichromosome maintenance 10 replication initiation factor
NCBI Official Symbol
MCM10
NCBI Official Synonym Symbols
CNA43; DNA43; PRO2249
NCBI Protein Information
protein MCM10 homolog
UniProt Protein Name
Protein MCM10 homolog
UniProt Gene Name
MCM10
UniProt Synonym Gene Names
HsMCM10
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Product Notes
The MCM10 mcm10 (Catalog #AAA322986) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCM10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCM10 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MCM10 mcm10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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