WB (Western Blot)
(Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 ug) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. AAA211140) used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)
Rabbit anti-Human MCM2 Polyclonal Antibody | anti-MCM2 antibody
Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MCM2, Antibody; Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified; MCM2, Phospho (S40/S41); D3S3194; MITOTIN; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); nuclear protein BM28; DFNA70; cell devision cycle-like 1; CDCL1; cdc19; CCNL1; BM28; DNA replication licensing factor MCM2; cyclin-like 1; minichromosome maintenance complex component 2; anti-MCM2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-MCM2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Surrounding serine 40/41
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 ug) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. AAA211140) used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)
WB (Western Blot)
(Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 ug) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. AAA211140) used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)
IHC (Immunohiostchemistry)
(Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. AAA211140 Lot 2) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. AAA211140 Lot 2) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.1 ug/ml anti-phospho-MCM2 (pS40/41) [AAA211140]in a 150 ul volume. DNA content was simultaneously analyzed via PI stain.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.1 ug/ml anti-phospho-MCM2 (pS40/41) [AAA211140]in a 150 ul volume. DNA content was simultaneously analyzed via PI stain.)
Related Product Information for anti-MCM2 antibody
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity.
Product Categories/Family for anti-MCM2 antibody
NCBI and Uniprot Product Information
UniProt Accession #
UniProt Protein Name
DNA replication licensing factor MCM2
UniProt Gene Name
MCM2
UniProt Synonym Gene Names
BM28; CCNL1; CDCL1; KIAA0030
UniProt Entry Name
MCM2_HUMAN
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Product Notes
The MCM2 mcm2 (Catalog #AAA211140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCM2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MCM2 mcm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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