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ICC (Immunocytochemistry)
(Anti-MCM2 antibody, AAA45126, ICCICC: NIH3T3 Cell)
Rabbit anti-Mouse, Rat MCM2 Polyclonal Antibody | anti-MCM2 antibody
Anti-MCM2 antibody
Gene Names
Mcm2; BM28; CDCL1; Mcmd2; AA959861; AW476101; mKIAA0030
Reactivity
Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
MCM2, Antibody; Anti-MCM2 antibody; DNA replication licensing factor MCM2; minichromosome maintenance complex component 2; BM28 antibody; CCNL 1 antibody; CCNL1 antibody; CDC like 1 antibody; CDC like-1 antibody; cdc19 antibody; CDCL 1 antibody; CDCL1 antibody; Cell devision cycle like 1 antibody; Cyclin like 1 antibody; cyclin like-1 antibody; D3S3194 antibody; DNA replication licensing factor MCM2 antibody; KIAA0030 antibody; MCM 2 antibody; MCM2 antibody; MCM2 minichromosome maintenance deficient 2 mitotin antibody; MCM2 minichromosome maintenance deficient 2 mitotin(S. cerevisiae) antibody; MCM2 minichromosome maintenance deficient 2, mitotin antibody; MCM2_HUMAN antibody; MGC10606 antibody; Minichromosome maintenance complex component 2 antibody; Minichromosome maintenance deficient 2(mitotin) antibody; Minichromosome maintenance deficient 2 mitotin antibody; Minichromosome maintenance protein 2 antibody; Minichromosome maintenance protein 2 homolog antibody; Mitotin antibody; Nuclear protein BM28 antibody; OTTHUMP00000216047 antibody; OTTHUMP00000216050 antibody; anti-MCM2 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
904
Applicable Applications for anti-MCM2 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2 (884-904aa DLFKFNKFSRDLKRKLILQQF), different from the related rat sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-MCM2 antibody, AAA45126, ICCICC: NIH3T3 Cell)
ICC (Immunocytochemistry)
(Anti-MCM2 antibody, AAA45126, ICCICC: NIH3T3 Cell)
ICC (Immunocytochemistry)
(Anti-MCM2 antibody, AAA45126, ICCICC: HEPA Cell)
ICC (Immunocytochemistry)
(Anti-MCM2 antibody, AAA45126, ICCICC: HEPA Cell)
WB (Western Blot)
(Anti-MCM2 antibody, AAA45126, Western blottingAll lanes: Anti MCM2 (AAA45126) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Mouse Intestine Tissue Lysate at 50ugLane 4: Mouse Testis Tissue Lysate at 50ugLane 5: Mouse Ovary Tissue Lysate at 50ugLane 6: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 102KDObserved bind size: 102KD)
WB (Western Blot)
(Anti-MCM2 antibody, AAA45126, Western blottingAll lanes: Anti MCM2 (AAA45126) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Mouse Intestine Tissue Lysate at 50ugLane 4: Mouse Testis Tissue Lysate at 50ugLane 5: Mouse Ovary Tissue Lysate at 50ugLane 6: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 102KDObserved bind size: 102KD)
IHC (Immunohiostchemistry)
(Anti-MCM2 antibody, AAA45126, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti-MCM2 antibody, AAA45126, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-MCM2 antibody, AAA45126, IHC(P)IHC(P): Rat Intestinal Lymphocyte Tissue)
IHC (Immunohistochemistry)
(Anti-MCM2 antibody, AAA45126, IHC(P)IHC(P): Rat Intestinal Lymphocyte Tissue)
Related Product Information for anti-MCM2 antibody
Description: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC in Mouse, Rat.
Background: MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
Background: MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
102,078 Da
NCBI Official Full Name
DNA replication licensing factor MCM2
NCBI Official Synonym Full Names
minichromosome maintenance deficient 2 mitotin (S. cerevisiae)
NCBI Official Symbol
Mcm2
NCBI Official Synonym Symbols
BM28; CDCL1; Mcmd2; AA959861; AW476101; mKIAA0030
NCBI Protein Information
DNA replication licensing factor MCM2; nuclear protein BM28; mini chromosome maintenance deficient 2; minichromosome maintenance protein 2 homolog
UniProt Protein Name
DNA replication licensing factor MCM2
UniProt Gene Name
Mcm2
UniProt Synonym Gene Names
Bm28; Cdcl1; Kiaa0030; Mcmd2
UniProt Entry Name
MCM2_MOUSE
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Product Notes
The MCM2 mcm2 (Catalog #AAA45126) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCM2 antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCM2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MCM2 mcm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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