IHC (Immunohistochemisry)
(MCM2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit MCM2 Polyclonal Antibody | anti-MCM2 antibody
MCM2 Antibody (C-term)
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, mouse (Predicted Reactivity: Mouse, Xenopus)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MCM2, Antibody; MCM2 Antibody (C-term); DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; MCM2; BM28; CCNL1; CDCL1; KIAA0030; anti-MCM2 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Mouse, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.42 (varies by lot)
Sequence Positions
649-677
Sequence Length
904
Applicable Applications for anti-MCM2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(MCM2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(MCM2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of MCM2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of MCM2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(All lanes : Anti-MCM2 Antibody (C-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 102 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-MCM2 Antibody (C-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 102 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MCM2 antibody
MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
References
Saade, E., et al. Proteomics 9(21):4934-4943(2009)
Xu, X., et al. EMBO J. 28(19):3005-3014(2009)
Liaw, K., et al. J. Cutan. Pathol. 36(10):1121-1122(2009)
Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)
Chuang, L.C., et al. Mol. Cell 35(2):206-216(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Xu, X., et al. EMBO J. 28(19):3005-3014(2009)
Liaw, K., et al. J. Cutan. Pathol. 36(10):1121-1122(2009)
Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)
Chuang, L.C., et al. Mol. Cell 35(2):206-216(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101896
NCBI Official Full Name
DNA replication licensing factor MCM2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 2
NCBI Official Symbol
MCM2
NCBI Official Synonym Symbols
BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
NCBI Protein Information
DNA replication licensing factor MCM2
UniProt Protein Name
DNA replication licensing factor MCM2
UniProt Gene Name
MCM2
UniProt Synonym Gene Names
BM28; CCNL1; CDCL1; KIAA0030
UniProt Entry Name
MCM2_HUMAN
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Product Notes
The MCM2 mcm2 (Catalog #AAA286163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 649-677. The MCM2 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Mouse, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's MCM2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MCM2 mcm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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