WB (Western Blot)
(Detection of human MCM4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM4 antibody AAA210775 (lot AAA210775-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Goat anti-Human, Mouse MCM4 Polyclonal Antibody | anti-MCM4 antibody
Goat anti-MCM4 Antibody, Affinity Purified
Gene Names
MCM4; NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MCM4, Antibody; Goat anti-MCM4 Antibody, Affinity Purified; CDC54; P1-CDC21; NKGCD; NKCD; minichromosome maintenance deficient 4; IMD54; homolog of S. pombe cell devision cycle 21; DNA replication licensing factor MCM4; CDC21 homolog; CDC21; hCdc21; minichromosome maintenance complex component 4; anti-MCM4 antibody
Host
Goat
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MCM4 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MCM4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM4 antibody AAA210775 (lot AAA210775-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human MCM4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM4 antibody AAA210775 (lot AAA210775-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohiostchemistry)
(Detection of mouse MCM4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-MCM4 (Cat. No. AAA210775 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse MCM4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-MCM4 (Cat. No. AAA210775 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MCM4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM4 (Cat. No. AAA210775 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MCM4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM4 (Cat. No. AAA210775 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-MCM4 antibody
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM4 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM4 is phosphorylated by CDC2 kinase resulting in a reduction of helicase activity and chromatin binding.
Product Categories/Family for anti-MCM4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,558 Da
NCBI Official Full Name
DNA replication licensing factor MCM4
NCBI Official Synonym Full Names
minichromosome maintenance complex component 4
NCBI Official Symbol
MCM4
NCBI Official Synonym Symbols
NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
NCBI Protein Information
DNA replication licensing factor MCM4; CDC21 homolog; minichromosome maintenance deficient 4; homolog of S. pombe cell devision cycle 21
UniProt Protein Name
DNA replication licensing factor MCM4
UniProt Gene Name
MCM4
UniProt Synonym Gene Names
CDC21
UniProt Entry Name
MCM4_HUMAN
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Product Notes
The MCM4 mcm4 (Catalog #AAA210775) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MCM4 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MCM4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MCM4 mcm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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