IHC (Immunohiostchemistry)
(MCM6 Antibody (N-term) (AAA287071)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCM6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit MCM6 Polyclonal Antibody | anti-MCM6 antibody
MCM6 Antibody (N-term)
Gene Names
MCM6; Mis5; P105MCM; MCG40308
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MCM6, Antibody; MCM6 Antibody (N-term); DNA replication licensing factor MCM6; p105MCM; MCM6; anti-MCM6 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MCM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human MCM6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
80-109
Sequence Length
821
Applicable Applications for anti-MCM6 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(MCM6 Antibody (N-term) (AAA287071)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCM6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(MCM6 Antibody (N-term) (AAA287071)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCM6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(MCM6 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MCM6 antibody detected the MCM6 protein (arrow).)
WB (Western Blot)
(MCM6 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MCM6 antibody detected the MCM6 protein (arrow).)
Related Product Information for anti-MCM6 antibody
The protein encoded by this gene is one of the highly
conserved mini-chromosome maintenance proteins (MCM) that are
essential for the initiation of eukaryotic genome replication. The
hexameric protein complex formed by the MCM proteins is a key
component of the pre-replication complex (pre_RC) and may be
involved in the formation of replication forks and in the
recruitment of other DNA replication related proteins. The MCM
complex consisting of this protein and MCM2, 4 and 7 proteins
possesses DNA helicase activity, and may act as a DNA unwinding
enzyme. The phosphorylation of the complex by CDC2 kinase reduces
the helicase activity, suggesting a role in the regulation of DNA
replication.
conserved mini-chromosome maintenance proteins (MCM) that are
essential for the initiation of eukaryotic genome replication. The
hexameric protein complex formed by the MCM proteins is a key
component of the pre-replication complex (pre_RC) and may be
involved in the formation of replication forks and in the
recruitment of other DNA replication related proteins. The MCM
complex consisting of this protein and MCM2, 4 and 7 proteins
possesses DNA helicase activity, and may act as a DNA unwinding
enzyme. The phosphorylation of the complex by CDC2 kinase reduces
the helicase activity, suggesting a role in the regulation of DNA
replication.
References
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Timpson, N.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1341-1348(2010)
Wei, Z., et al. J. Biol. Chem. 285(17):12469-12473(2010)
Upton, J., et al. N. Z. Med. J. 123 (1308), 123 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Timpson, N.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1341-1348(2010)
Wei, Z., et al. J. Biol. Chem. 285(17):12469-12473(2010)
Upton, J., et al. N. Z. Med. J. 123 (1308), 123 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92889
NCBI Official Full Name
DNA replication licensing factor MCM6
NCBI Official Synonym Full Names
minichromosome maintenance complex component 6
NCBI Official Symbol
MCM6
NCBI Official Synonym Symbols
Mis5; P105MCM; MCG40308
NCBI Protein Information
DNA replication licensing factor MCM6
UniProt Protein Name
DNA replication licensing factor MCM6
UniProt Gene Name
MCM6
UniProt Entry Name
MCM6_HUMAN
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Product Notes
The MCM6 mcm6 (Catalog #AAA287071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 80-109. The MCM6 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's MCM6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MCM6 mcm6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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