FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit MDH2 Polyclonal Antibody | anti-MDH2 antibody
MDH2 Antibody (Center)
Gene Names
MDH2; MDH; MOR1; M-MDH; MGC:3559
Reactivity
Human, mouse (Predicted Reactivity: Yeast, Bovine, C Elegans, Monkey, Pig, Rat)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MDH2, Antibody; MDH2 Antibody (Center); Malate dehydrogenase; mitochondrial; MDH2; anti-MDH2 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Yeast, Bovine, C Elegans, Monkey, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the Central region of human MDH2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
86-115
Sequence Length
296
Applicable Applications for anti-MDH2 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Cellular location
Mitrochondrion matrix {ECO:0000250}|UniprotKB:P04636
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8° for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(MDH2 Antibody (Center) western blot analysis in A431,Hela and mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the MDH2 antibody detected the MDH2 protein (arrow).)
WB (Western Blot)
(MDH2 Antibody (Center) western blot analysis in A431,Hela and mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the MDH2 antibody detected the MDH2 protein (arrow).)
Related Product Information for anti-MDH2 antibody
MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Product Categories/Family for anti-MDH2 antibody
References
Rzem,R.,et.al., J. Inherit. Metab. Dis. 30 (5), 681-689 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35503
NCBI Official Full Name
malate dehydrogenase, mitochondrial isoform 2
NCBI Official Synonym Full Names
malate dehydrogenase 2, NAD (mitochondrial)
NCBI Official Symbol
MDH2
NCBI Official Synonym Symbols
MDH; MOR1; M-MDH; MGC:3559
NCBI Protein Information
malate dehydrogenase, mitochondrial
UniProt Protein Name
Malate dehydrogenase, mitochondrial
UniProt Gene Name
MDH2
UniProt Entry Name
MDHM_HUMAN
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Product Notes
The MDH2 mdh2 (Catalog #AAA289831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 86-115. The MDH2 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Yeast, Bovine, C Elegans, Monkey, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's MDH2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MDH2 mdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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