IHC (Immunohiostchemistry)
(AAA323116 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit mDia1 Polyclonal Antibody | anti-mDia1 antibody
mDia1 Antibody
Gene Names
DIAPH1; DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
mDia1, Antibody; mDia1 Antibody; DIAPH1; deafness, autosomal dominant 1; DFNA1; DIA1; DIAP1; DIAP1_HUMAN; Diaphanous homolog 1 (Drosophila); diaphanous homolog 1; Diaphanous related formin 1; Diaphanous-related formin-1; DRF1; FLJ25265; hDIA1; LFHL1; low frequency hearing loss 1; p140DIA; Protein diaphanous homolog 1; anti-mDia1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
mDia1 antibody detects endogenous levels of mDia1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1263
Applicable Applications for anti-mDia1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human mDia1.
Subcellular Location
Cell Membrane. Cell Projection > Ruffle Membrane. Cytoplasm > Cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
Tissue Specificity
Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets (PubMed:26912466).
Predicted Cross Reactivity
Horse, Sheep, Rabbit, Dog
Similarity
Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA323116 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323116 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using mDia1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using mDia1 antibody.)
Related Product Information for anti-mDia1 antibody
Function: Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed:20937854, PubMed:21834987). Plays a role in brain development (PubMed:24781755).
Subunit Structure: Homodimer. Interacts with the GTP-bound form of RHOA. Interacts with RHOC, PFY1, MAPRE1, BAIAP2 and APC. Interacts with SCAI (By similarity). Interacts with DCAF7, via FH2 domain. Interacts with NCDN (By similarity). Interacts with RHOA, OSBPL10, OSBPL2, VIM, TUBB and DYN1 (PubMed:23325789).
Post-translational Modifications: Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement.
Similarity: The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity). Belongs to the formin homology family. Diaphanous subfamily.
Subunit Structure: Homodimer. Interacts with the GTP-bound form of RHOA. Interacts with RHOC, PFY1, MAPRE1, BAIAP2 and APC. Interacts with SCAI (By similarity). Interacts with DCAF7, via FH2 domain. Interacts with NCDN (By similarity). Interacts with RHOA, OSBPL10, OSBPL2, VIM, TUBB and DYN1 (PubMed:23325789).
Post-translational Modifications: Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement.
Similarity: The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity). Belongs to the formin homology family. Diaphanous subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 140-150; 70 kDa
Predicted: 142 kDa
Predicted: 142 kDa
NCBI Official Full Name
protein diaphanous homolog 1 isoform 2
NCBI Official Synonym Full Names
diaphanous related formin 1
NCBI Official Symbol
DIAPH1
NCBI Official Synonym Symbols
DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1
NCBI Protein Information
protein diaphanous homolog 1
UniProt Protein Name
Protein diaphanous homolog 1
UniProt Gene Name
DIAPH1
UniProt Synonym Gene Names
DIAP1; DRF1
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Product Notes
The mDia1 diaph1 (Catalog #AAA323116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mDia1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's mDia1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the mDia1 diaph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mDia1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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