WB (Western Blot)
(HEK293 overexpressing MDM2 (RC219518) and probed (mock transfection in first lane), tested by Origene.)
Goat MDM2 Polyclonal Antibody | anti-MDM2 antibody
Goat anti-MDM2 (isoform) Antibody
Gene Names
MDM2; HDMX; hdm2; ACTFS
Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Cat, Dog, Pig, Cow
Applications
Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MDM2, Antibody; Goat anti-MDM2 (isoform) Antibody; MDM2; Mdm2; transformed 3T3 cell double minute 2; p53 binding protein (mouse); HGNC: 6973; MGC71221; hdm2; mouse double minute 2 homolog; mouse double minute 2, human homolog of; p53-binding protein; p53-binding protein MDM2; ubiquitin-protein ligase E3 Md; MDM2 antibody; Mdm2 antibody; transformed 3T3 cell double minute 2 antibody; p53 binding protein (mouse) antibody; HGNC: 6973 antibody; MGC71221 antibody; hdm2 antibody; mouse double minute 2 homolog antibody; mouse double minute 2; human homolog of antibody; p53-binding protein antibody; p53-binding protein MDM2 antibody; ubiquitin-protein ligase E3 Mdm2 antibody; Mdm2 p53 binding protein homolog (mouse) antibody; HDMX antibody; MGC5370 antibody; p53 binding protein antibody; OTTHUMP00000183495 antibody; double minute 2; MDM2 (isoform); anti-MDM2 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Cat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DELSGERQRKRHKSD
Sequence Length
497
Applicable Applications for anti-MDM2 antibody
WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-DELSGERQRKRHKSD, from the internal region of the protein sequence according to NP_002383.2.
Epitope
Internal region
Note
This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
(HEK293 overexpressing MDM2 (RC219518) and probed (mock transfection in first lane), tested by Origene.)
WB (Western Blot)
(HEK293 overexpressing MDM2 (RC219518) and probed (mock transfection in first lane), tested by Origene.)
WB (Western Blot)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,991 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase Mdm2 isoform a
NCBI Official Synonym Full Names
MDM2 proto-oncogene
NCBI Official Symbol
MDM2
NCBI Official Synonym Symbols
HDMX; hdm2; ACTFS
NCBI Protein Information
E3 ubiquitin-protein ligase Mdm2
UniProt Protein Name
E3 ubiquitin-protein ligase Mdm2
UniProt Gene Name
MDM2
UniProt Synonym Gene Names
Hdm2
UniProt Entry Name
MDM2_HUMAN
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Product Notes
The MDM2 mdm2 (Catalog #AAA61023) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MDM2 (isoform) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat, Cat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MDM2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the MDM2 mdm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DELSGERQRK RHKSD. It is sometimes possible for the material contained within the vial of "MDM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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