Rabbit MeCP2 Polyclonal Antibody | anti-MECP2 antibody

MeCP2 Antibody (N-term S80)

Cat. Num. AAA285734

• Gene Names: MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• Reactivity: Human (Predicted Reactivity: Monkey, Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MeCP2, Antibody; MeCP2 Antibody (N-term S80); Methyl-CpG-binding protein 2; MeCp-2 protein; MeCp2; MECP2; anti-MECP2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human MeCP2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 58-87
• Sequence Length: 498

• Applicable Applications for anti-MECP2 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).)

WB (Western Blot)

(Anti-MeCP2 Antibody (N-term S80) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MECP2 antibody

DNA methylation is the major modification of eukaryotic
genomes and plays an essential role in mammalian development. Human
proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG
binding domain (MBD). Each of these proteins, with the exception of
MBD3, is capable of binding specifically to methylated DNA. MECP2,
MBD1 and MBD2 can also repress transcription from methylated gene
promoters. In contrast to other MBD family members, MECP2 is
X-linked and subject to X inactivation. MECP2 is dispensible in
stem cells, but is essential for embryonic development. MECP2 gene
mutations are the cause of most cases of Rett syndrome, a
progressive neurologic developmental disorder and one of the most
common causes of mental retardation in females. [provided by
RefSeq].

Product Categories/Family for anti-MECP2 antibody

Neuroscience

References

Shapiro, J.R., et al. Pediatr. Res. 68(5):446-451(2010)
Pintaudi, M., et al. Epilepsy Behav (2010) In press :
Jain, D., et al. Pediatr. Neurol. 43(1):35-40(2010)
Harvey, C.G., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 144B (3), 355-360 (2007) :
Francke, U. Nat Clin Pract Neurol 2(4):212-221(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 160707950
• NCBI GeneID: 4204
• NCBI Accession #: NP_001104262.1; NP_004983.1
• NCBI GenBank Nucleotide #: NM_001110792.1
• UniProt Accession #: P51608; O15233; Q6QHH9; Q7Z384
• Molecular Weight: 52441
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 2
• NCBI Official Synonym Full Names: methyl CpG binding protein 2
• NCBI Official Symbol: MECP2
• NCBI Official Synonym Symbols: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• NCBI Protein Information: methyl-CpG-binding protein 2
• UniProt Protein Name: Methyl-CpG-binding protein 2
• UniProt Gene Name: MECP2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2
• UniProt Entry Name: MECP2_HUMAN