IHC (Immunohistochemisry)
(MED25 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit MED25 Polyclonal Antibody | anti-MED25 antibody
MED25 Antibody (N-term)
Gene Names
MED25; P78; ACID1; ARC92; PTOV2; CMT2B2; TCBAP0758
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MED25, Antibody; MED25 Antibody (N-term); Mediator of RNA polymerase II transcription subunit 25; Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC92; Mediator complex subunit 25; p78; MED25; ACID1; PTOV2; anti-MED25 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human MED25.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
112-140
Sequence Length
747
Applicable Applications for anti-MED25 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(MED25 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(MED25 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(MED25 Antibody (N-term) ( western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).)
WB (Western Blot)
(MED25 Antibody (N-term) ( western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).)
WB (Western Blot)
(MED25 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).)
WB (Western Blot)
(MED25 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).)
Related Product Information for anti-MED25 antibody
This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].
References
Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010) : Leal, A., et al. Neurogenetics 10(4):275-287(2009) Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007) Sato, S., et al. Mol. Cell 14(5):685-691(2004) Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78171
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 25
NCBI Official Synonym Full Names
mediator complex subunit 25
NCBI Official Symbol
MED25
NCBI Official Synonym Symbols
P78; ACID1; ARC92; PTOV2; CMT2B2; TCBAP0758
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 25
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 25
UniProt Gene Name
MED25
UniProt Synonym Gene Names
ACID1; ARC92; PTOV2; ARC92
UniProt Entry Name
MED25_HUMAN
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Product Notes
The MED25 med25 (Catalog #AAA288279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 112-140. The MED25 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's MED25 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MED25 med25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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