Rabbit MEK1/2 Polyclonal Antibody | anti-MAP2K1 antibody

MEK1/2 Antibody

Cat. Num. AAA40844

• Gene Names: MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: MEK1/2, Antibody; MEK1/2 Antibody; Mitogen-activated protein kinase kinase 1, MAP2K1, MAPKK1, MEK1, MKK1, PRKMK1, CFC3, Mitogen-activated protein kinase kinase 2, MAP2K2, MAPKK2, MEK2, MKK2, PRKMK2, CFC; anti-MAP2K1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MEK1/2 antibody is human, mouse, and rat reactive. This antibody detects both MEK1 and MEK2.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-MAP2K1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: Human Brain Tissue Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-MAP2K1 antibody

The mitogen-activated protein kinase kinase 1 (MAP2K1, also known as MEK1) and the highly homologous MEK2 are members of the dual specificity protein kinase family (1). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals (2). MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (2).

Product Categories/Family for anti-MAP2K1 antibody

Signal Transduction

References

Zheng CF and Guan KL. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 1993; 268:11435-9. Marais R and Marshall CJ. Control of the ERK MAP kinase cascade by Ras and Raf. Cancer Surv. 1996; 27:101-25.

NCBI and Uniprot Product Information

• NCBI GI #: 5579478
• NCBI GeneID: 5604
• NCBI Accession #: NP_002746
• NCBI GenBank Nucleotide #: NM_002755.3
• UniProt Accession #: Q0VD16; F2Z4G4; Q02750
• Molecular Weight: Predicted: 43 kDa; Observed: 44 kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: MAP2K1 protein
• UniProt Gene Name: MAP2K1
• UniProt Entry Name: Q0VD16_BOVIN