WB (Western Blot)
(Anti-MESDC2 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:25 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human MESDC2 Polyclonal Antibody | anti-MESD antibody
Anti-MESDC2 Antibody, Rabbit Polyclonal
Gene Names
MESDC2; BOCA; MESD
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MESDC2, Antibody; Anti-MESDC2 Antibody, Rabbit Polyclonal; MESDC2/MESD Antibody, Rabbit PAb, Antigen Affinity Purified; mesoderm development candidate 2; Anti-BOCA Antibody; Anti-MESD Antibody; anti-MESD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MESDC2/MESD
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MESD antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Recombinant Human MESD protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human MESDC2 (rh MESDC2). MESDC2 specific IgG was purified by human MESDC2 affinity chromatography
.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-MESDC2 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:25 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-MESDC2 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:25 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(MESDC2 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg NIH-3T3 Whole Cell Lysate2 uL anti-MESDC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MESDC2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)
IP (Immunoprecipitation)
(MESDC2 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg NIH-3T3 Whole Cell Lysate2 uL anti-MESDC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MESDC2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)
Related Product Information for anti-MESD antibody
MESD (Mesoderm Development LRP Chaperone) is a Protein Coding gene. LDLR chaperone MESD, also known as Mesoderm development protein, Mesoderm development candidate 2, Renal carcinoma antigen NY-REN-61, and MESDC2, is a member of the MESD family. It is widely expressed in the thyroid, placenta, and other tissues. MESDC2 is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). The LDLR maturation activity resides in the N-and C-terminal unstructured regions. MESDC2 acts as a modulator of the Wnt pathway since some LDLRs are coreceptors for the canonical Wnt pathway. MESDC2 is essential for the specification of embryonic polarity and mesoderm induction.
References
Scanlan M.J. et al., 1999, Int. J. Cancer 83:456-464. Veltman, IM. et al., 2005, Hum Mol Genet 14 (14):1955-63. Koduri V. et al., 2007, Biochemistry 46:6570-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,895 Da
NCBI Official Full Name
LDLR chaperone MESD
NCBI Official Synonym Full Names
mesoderm development candidate 2
NCBI Official Symbol
MESDC2
NCBI Official Synonym Symbols
BOCA; MESD
NCBI Protein Information
LDLR chaperone MESD; mesoderm development protein; renal carcinoma antigen NY-REN-61
UniProt Protein Name
LDLR chaperone MESD
UniProt Gene Name
MESDC2
UniProt Synonym Gene Names
KIAA0081; MESD
UniProt Entry Name
MESD_HUMAN
Similar Products
Product Notes
The MESD mesdc2 (Catalog #AAA255262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MESDC2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MESDC2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MESD mesdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MESDC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.