Rabbit anti-Human Metabotropic Glutamate Receptor 1 Polyclonal Antibody | anti-GRM1 antibody

Metabotropic Glutamate Receptor 1 Antibody (C-term)

Cat. Num. AAA290595

• Gene Names: GRM1; MGLU1; GPRC1A; MGLUR1; SCAR13; PPP1R85
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Metabotropic Glutamate Receptor 1, Antibody; Metabotropic Glutamate Receptor 1 Antibody (C-term); Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab); Metabotropic glutamate receptor 1; mGluR1; GRM1; GPRC1A; MGLUR1; anti-GRM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Metabotropic Glutamate Receptor 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1096-1126 amino acids from the C-terminal region of human Metabotropic Glutamate Receptor 1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1194

• Applicable Applications for anti-GRM1 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Antigen Region: 1096-1126
• Antigen Source: Human
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(GPRC1A Antibody(Y1111) western blot analysis in CEM,NCI-H292,NCI-H460 cell line lysates (35ug/lane).This demonstrates the GPRC1A antibody detected the GPRC1A protein (arrow).)

IHC (Immunohiostchemistry)

(Metabotropic Glutamate Receptor 1 (GPRC1A) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Metabotropic Glutamate Receptor 1 (GPRC1A) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Anti-GPRC1A Antibody (Y1111) at 1:1000 dilution + human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 132 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-GRM1 antibody

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 (also known as GPRC1A) and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The activity of GRM1 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. This protein may participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum

Product Categories/Family for anti-GRM1 antibody

Neuroscience; GPCR Antibodies

References

Hlavackova, V., et al., EMBO J. 24(3):499-509 (2005). Kammermeier, P.J., et al., J. Pharmacol. Exp. Ther. 312(2):502-508 (2005). Burgueno, J., et al., Exp. Cell Res. 300(1):23-34 (2004). Mundell, S.J., et al., Mol. Pharmacol. 65(6):1507-1516 (2004). Anneser, J.M., et al., J. Neuropathol. Exp. Neurol. 63(8):831-840 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 493798972
• NCBI GeneID: 2911
• NCBI Accession #: NP_001264993.1; NP_001264994.1; NP_001264995.1; NP_001264996.1
• NCBI GenBank Nucleotide #: NM_001278064.1
• UniProt Accession #: Q13255; Q13256; Q14757; Q14758; Q5VTF7; Q5VTF8; Q9NU10; Q9UGS9; Q9UGT0; B9EG79; F8W805
• Molecular Weight: 132357
• NCBI Official Full Name: metabotropic glutamate receptor 1 isoform alpha
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 1
• NCBI Official Symbol: GRM1
• NCBI Official Synonym Symbols: MGLU1; GPRC1A; MGLUR1; SCAR13; PPP1R85
• NCBI Protein Information: metabotropic glutamate receptor 1
• UniProt Protein Name: Metabotropic glutamate receptor 1
• UniProt Gene Name: GRM1
• UniProt Synonym Gene Names: GPRC1A; MGLUR1; mGluR1
• UniProt Entry Name: GRM1_HUMAN