Rabbit MFAP3 Polyclonal Antibody | anti-MFAP3 antibody

Anti-MFAP3 Antibody Picoband

Cat. Num. AAA19989

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: MFAP3, Antibody; Anti-MFAP3 Antibody Picoband; Follicular dendritic cell secreted peptide; RNA-binding protein 47; RNA-binding motif protein 47; RBM47; anti-MFAP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-MFAP3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml, Human; IHC(Paraffin-embedded Section): 2-5ug/ml, Human, Mouse, Rat; FC/FACS (Fixed): 1-3ug/1x10⁶ cells, Human; ELISA: 0.1-0.5ug/ml
• Immunogen: E coli-derived human MFAP3 recombinant protein (Position: A19-N349).
• Subcellular Localization: Nucleus
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross-reactivity with other proteins
• Protein Function: Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 6. Flow Cytometry analysis of 293T cells using anti-MFAP3 antibody (AAA19989).Overlay histogram showing 293T cells stained with AAA19989 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MFAP3 Antibody (AAA19989, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of MFAP3 using anti-MFAP3 antibody (AAA19989).MFAP3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFAP3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MFAP3 at approximately 45-50 kDa. The expected band size for MFAP3 is at 40 kDa.)

Related Product Information for anti-MFAP3 antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-MFAP3 antibody

Primary Antibodies; Chromatin Binding Proteins; DNA/RNA Binding; Epigenetics and Nuclear Signaling

References

1. Abrams, W. R., Ma, R.-I., Kucich, U., Bashir, M. M., Decker, S., Tsipouras, P., McPherson, J. D., Wasmuth, J. J., Rosenbloom, J. Molecular cloning of the microfibrillar protein MFAP3 and assignment of the gene to human chromosome 5q32-q33.2. Genomics 26: 47-54, 1995.

NCBI and Uniprot Product Information

• NCBI GI #: 334191695
• NCBI GeneID: 4238
• NCBI Accession #: NP_001229265
• NCBI GenBank Nucleotide #: NM_001242336.1
• UniProt Accession #: P55082; Q9NXA7; B2RDK0; B4DKA1
• Molecular Weight: 16.7 kDa (152aa) confirmed by MALDI-TOF
• NCBI Official Full Name: microfibril-associated glycoprotein 3 isoform 1
• NCBI Official Synonym Full Names: microfibril associated protein 3
• NCBI Official Symbol: MFAP3
• NCBI Protein Information: microfibril-associated glycoprotein 3
• UniProt Protein Name: Microfibril-associated glycoprotein 3
• UniProt Gene Name: MFAP3