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WB (Western Blot)
(Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term) (AAA285191). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.)
Rabbit MFAP5 Polyclonal Antibody | anti-MFAP5 antibody
MFAP5 Antibody (C-term)
Gene Names
MFAP5; AAT9; MP25; MAGP2; MAGP-2; MFAP-5
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MFAP5, Antibody; MFAP5 Antibody (C-term); Microfibrillar-associated protein 5; MFAP-5; MP25; Microfibril-associated glycoprotein 2; MAGP-2; MFAP5; MAGP2; anti-MFAP5 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
133-160
Applicable Applications for anti-MFAP5 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed: ).
Calculated MW
19612
Cellular Location
Secreted, extracellular space, extracellular matrix
Preparation and Storage
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term) (AAA285191). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.)
WB (Western Blot)
(Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term) (AAA285191). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.)
FCM/FACS (Flow Cytometry)
(MFAP5 Antibody (C-term) (AAA285191) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MFAP5 Antibody (C-term) (AAA285191) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of MFAP5 antibody (C-term) (AAA285191) in K562 cell line lysates (35ug/lane). MFAP5 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of MFAP5 antibody (C-term) (AAA285191) in K562 cell line lysates (35ug/lane). MFAP5 (arrow) was detected using the purified Pab.)
Related Product Information for anti-MFAP5 antibody
MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
Product Categories/Family for anti-MFAP5 antibody
References
Albig,A.R., Becenti,D.J. Microvasc. Res. 76 (1), 7-14 (2008)
Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006)
Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002)
Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)
Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006)
Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002)
Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
microfibrillar-associated protein 5 isoform a
NCBI Official Synonym Full Names
microfibrillar associated protein 5
NCBI Official Symbol
MFAP5
NCBI Official Synonym Symbols
AAT9; MP25; MAGP2; MAGP-2; MFAP-5
NCBI Protein Information
microfibrillar-associated protein 5
UniProt Protein Name
Microfibrillar-associated protein 5
UniProt Gene Name
MFAP5
UniProt Synonym Gene Names
MAGP2; MFAP-5; MAGP-2
UniProt Entry Name
MFAP5_HUMAN
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Product Notes
The MFAP5 mfap5 (Catalog #AAA285191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 133-160. The MFAP5 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MFAP5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MFAP5 mfap5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MFAP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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