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FCM/FACS (Flow Cytometry)
(MFRN2 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human MFRN2 Polyclonal Antibody | anti-SLC25A28 antibody
MFRN2 Antibody (N-term)
Gene Names
SLC25A28; MFRN2; MRS4L; MRS3/4; NPD016
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
MFRN2, Antibody; MFRN2 Antibody (N-term); Mitoferrin-2; Mitochondrial RNA-splicing protein 3/4 homolog; MRS3/4; hMRS3/4; Mitochondrial iron transporter 2; Solute carrier family 25 member 28; SLC25A28; MFRN2; anti-SLC25A28 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This MFRN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-64 amino acids from the N-terminal region of human MFRN2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
36-64
Sequence Length
364
Applicable Applications for anti-SLC25A28 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Mitochondrion inner membrane; Multi-pass membrane protein. Note=Isoform 1 and isoform 2 are both localized in the mitochondrion
Tissue Location
Ubiquitous. Expressed in placenta, lung, kidney, pancreas, liver, brain, skeletal muscle and heart
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(MFRN2 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MFRN2 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(MFRN2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(MFRN2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Anti-MFRN2 Antibody (N-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-MFRN2 Antibody (N-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Western blot analysis of MFRN2 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of MFRN2 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-SLC25A28 antibody
MFRN2 is mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme.
Product Categories/Family for anti-SLC25A28 antibody
References
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Deloukas, P., et al. Nature 429(6990):375-381(2004)
Wistow, G., et al. Mol. Vis. 8, 185-195 (2002)
Deloukas, P., et al. Nature 429(6990):375-381(2004)
Wistow, G., et al. Mol. Vis. 8, 185-195 (2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 39272
NCBI Official Full Name
mitoferrin-2
NCBI Official Synonym Full Names
solute carrier family 25 (mitochondrial iron transporter), member 28
NCBI Official Symbol
SLC25A28
NCBI Official Synonym Symbols
MFRN2; MRS4L; MRS3/4; NPD016
NCBI Protein Information
mitoferrin-2
UniProt Protein Name
Mitoferrin-2
UniProt Gene Name
SLC25A28
UniProt Synonym Gene Names
MFRN2; MRS3/4; hMRS3/4
UniProt Entry Name
MFRN2_HUMAN
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Product Notes
The SLC25A28 slc25a28 (Catalog #AAA288765) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 36-64. The MFRN2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MFRN2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SLC25A28 slc25a28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MFRN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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