Rabbit anti-Human MICA Polyclonal Antibody | anti-MICA antibody

MICA Antibody (Center)

Cat. Num. AAA28710

• Gene Names: MICA; MIC-A; PERB11.1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MICA, Antibody; MICA Antibody (Center); MHC class I polypeptide-related sequence A; MIC-A; MICA {ECO:0000312|EMBL:CAI419071}; anti-MICA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.42 (varies by lot)
• Sequence Positions: 68-97
• Sequence Length: 383

• Applicable Applications for anti-MICA antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Flow cytometric analysis of SK-BR-3 cells using MICA Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). AAA28710 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

WB (Western Blot)

(Anti-MICA Antibody (Center)at 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-MICA Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: Daudi whole cell lysatesLane 3: A431 whole cell lysatesLane 4: MCF-7 whole cell lysatesLane 5: U-87MG whole cell lysatesLane 6: THP-1 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-MICA Antibody (Center)at 1:2000 dilution + A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-MICA Antibody (Center) at 1:2000 dilutionLane 1: U-87 MG whole cell lysatesLane 2: A431 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-MICA Antibody (Center)at 1:2000 dilution + U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-MICA Antibody (Center) at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MICA antibody

MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Product Categories/Family for anti-MICA antibody

Immunology

References

Bahram,S., et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6259-6263 (1994) Klein,J.et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6251-6252 (1994) Parham,P., et.al., J. Immunol. 142 (11), 3937-3950 (1989)

NCBI and Uniprot Product Information

• NCBI GI #: 4557751
• NCBI GeneID: 100507436
• NCBI Accession #: NP_000238.1
• NCBI GenBank Nucleotide #: NM_000247.2
• UniProt Accession #: Q29983; O60917; O60918; O60919; O60920; O60933; O77956; O78150; A8KQR0; A9LRW5; A9QVE0; B1VJF1
• Molecular Weight: 42915
• NCBI Official Full Name: MHC class I polypeptide-related sequence A isoform 1 (MICA*001)
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence A
• NCBI Official Symbol: MICA
• NCBI Official Synonym Symbols: MIC-A; PERB11.1
• NCBI Protein Information: MHC class I polypeptide-related sequence A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: MIC-A
• UniProt Entry Name: MICA_HUMAN