Rabbit MID1 Polyclonal Antibody | anti-MID1 antibody

MID1 Polyclonal Antibody

Cat. Num. AAA28180

• Gene Names: MID1; OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity Purification
• Synonyms: MID1, Antibody; MID1 Polyclonal Antibody; MID1; BBBG1; FXY; GBBB1; MIDIN; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY; midline 1; anti-MID1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-MID1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500 - 1:2000; IF: 1:50 - 1:100
• Cross-Reactivity: Human
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1).
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of MCF-7 cells using MID1 antibody (AAA28180). Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of HeLa cells, using MID1 Rabbit pAb (AAA28180) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using MID1 antibody (AAA28180) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Related Product Information for anti-MID1 antibody

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.

Product Categories/Family for anti-MID1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 22653810
• NCBI GeneID: 4281
• UniProt Accession #: O15344; O75361; Q9BZX5; B2RCG2
• Molecular Weight: Calculated MW: 75kDa; Observed MW: 75kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase Midline-1
• NCBI Official Synonym Full Names: midline 1
• NCBI Official Symbol: MID1
• NCBI Official Synonym Symbols: OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
• NCBI Protein Information: E3 ubiquitin-protein ligase Midline-1; Opitz/BBB syndrome; RING finger protein 59; RING finger protein Midline-1; midline 1 RING finger protein; tripartite motif protein TRIM18; putative transcription factor XPRF; tripartite motif-containing protein 18; zinc finger on X and Y, mouse, homolog of
• UniProt Protein Name: E3 ubiquitin-protein ligase Midline-1
• UniProt Gene Name: MID1
• UniProt Synonym Gene Names: FXY; RNF59; TRIM18; XPRF
• UniProt Entry Name: TRI18_HUMAN