WB (Western Blot)
(Anti-MIF rabbit polyclonal antibody at 1:500 dilutionLane A: U937 Whole Cell LysateLane B: HL60 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: Raw264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:12 kDaObserved band size:12 kDa)
Rabbit anti-Mouse MIF Polyclonal Antibody | anti-MIF antibody
Anti-MIF Antibody, Rabbit Polyclonal
Gene Names
Mif; GIF; Glif
Reactivity
Mouse
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MIF, Antibody; Anti-MIF Antibody, Rabbit Polyclonal; MIF Antibody, Rabbit PAb, Antigen Affinity Purified; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Anti-GIF Antibody; Anti-Glif Antibody; anti-MIF antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse MIF
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MIF antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse MIF protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse MIF. MIF specific IgG was purified by Mouse MIF affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-MIF rabbit polyclonal antibody at 1:500 dilutionLane A: U937 Whole Cell LysateLane B: HL60 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: Raw264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:12 kDaObserved band size:12 kDa)
WB (Western Blot)
(Anti-MIF rabbit polyclonal antibody at 1:500 dilutionLane A: U937 Whole Cell LysateLane B: HL60 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: Raw264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:12 kDaObserved band size:12 kDa)
IP (Immunoprecipitation)
(Mouse MIF was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-Mouse MIF rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse MIF rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 12 kDaObserved band size: 12 kDa)
IP (Immunoprecipitation)
(Mouse MIF was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-Mouse MIF rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse MIF rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 12 kDaObserved band size: 12 kDa)
Related Product Information for anti-MIF antibody
Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is a ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of the central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread, and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important role in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.
References
Ohkawara T, et al. (2005) Pathophysiological roles of macrophage migration inhibitory factor in gastrointestinal, hepatic, and pancreatic disorders. J Gastroenterol. 40(2): 117-22.Bach JP, et al.. (2009) The role of macrophage inhibitory factor in tumorigenesis and central nervous system tumors. Cancer. 115(10): 2031-40.Rendon BE, et al.. (2009) Mechanisms of macrophage migration inhibitory factor (MIF)-dependent tumor microenvironmental adaptation. Exp Mol Pathol. 86(3): 180-5.Grieb G, et al. (2010) Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug News Perspect. 23(4): 257-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,504 Da
NCBI Official Full Name
macrophage migration inhibitory factor
NCBI Official Synonym Full Names
macrophage migration inhibitory factor
NCBI Official Symbol
Mif
NCBI Official Synonym Symbols
GIF; Glif
NCBI Protein Information
macrophage migration inhibitory factor
UniProt Protein Name
Macrophage migration inhibitory factor
UniProt Gene Name
Mif
UniProt Synonym Gene Names
MIF; DER6; GIF
UniProt Entry Name
MIF_MOUSE
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Product Notes
The MIF mif (Catalog #AAA257075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MIF Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MIF can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MIF mif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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