IHC (Immunohiostchemistry)
(Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, AAA45488, IHC(P)IHC(P): Human Prostatic Cancer Tissue)
Rabbit anti-Human, Rat Mitochondrial Pyruvate dehydrogenase kinase 1 Polyclonal Antibody | anti-PDK1 antibody
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Mitochondrial Pyruvate dehydrogenase kinase 1, Antibody; Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; pyruvate dehydrogenase kinase, isozyme 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 antibody; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody; HGNC:8809 antibody; mitochondrial antibody; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody; PDH kinase 1 antibody; Pdk1 antibody; PDK1_HUMAN antibody; Pyruvate dehydrogenase kinase isoform 1 antibody; Pyruvate dehydrogenase kinase, isoenzyme 1 antibody; anti-PDK1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Sequence Length
436
Applicable Applications for anti-PDK1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1 (408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, AAA45488, IHC(P)IHC(P): Human Prostatic Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, AAA45488, IHC(P)IHC(P): Human Prostatic Cancer Tissue)
WB (Western Blot)
(Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, AAA45488, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Skeletal Muscle Tissue LysateLane 3: HELA Cell LysateLane 4: M231 Cell LysateLane 5: COLO320 Cell LysateLane 6: SW620 Cell Lysate)
WB (Western Blot)
(Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, AAA45488, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Skeletal Muscle Tissue LysateLane 3: HELA Cell LysateLane 4: M231 Cell LysateLane 5: COLO320 Cell LysateLane 6: SW620 Cell Lysate)
Related Product Information for anti-PDK1 antibody
Description: Rabbit IgG polyclonal antibody for[Pyruvate dehydrogenase[lipoamide]] kinase isozyme 1, mitochondrial(PDK1) detection. Tested with WB, IHC-P in Human, Rat.
Background: PDK1(Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
Background: PDK1(Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
NCBI Official Synonym Full Names
pyruvate dehydrogenase kinase, isozyme 1
NCBI Official Symbol
PDK1
NCBI Protein Information
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; PDH kinase 1; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
UniProt Protein Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
UniProt Gene Name
PDK1
UniProt Synonym Gene Names
PDHK1; PDH kinase 1
UniProt Entry Name
PDK1_HUMAN
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Product Notes
The PDK1 pdk1 (Catalog #AAA45488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mitochondrial Pyruvate dehydrogenase kinase 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PDK1 pdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitochondrial Pyruvate dehydrogenase kinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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