Rabbit anti-Mouse Mki67 Polyclonal Antibody | anti-Mki67 antibody

Mki67 Rabbit pAb

Cat. Num. AAA28624

• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity purification
• Synonyms: Mki67, Antibody; Mki67 Rabbit pAb; Ki67; Ki-67; D630048A14Rik; anti-Mki67 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
• Sequence: DETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTP

• Applicable Applications for anti-Mki67 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: IHC-P: 1:500-1:1000; IF/ICC: 1:50-1:200; ELISA: Recommended starting concentration is 1ug/mL.; Please optimize the concentration based on your specific assay requirements.
• Cross Reactivity: Mouse, Rat
• Immunogen: Recombinant Protein corresponding to a sequence within amino acids 1816-2163 of mouse Mki67 (NP_001074586.2).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of paraffin-embedded Rat spleen tissue using Mouse Mki67 Rabbit pAb (AAA28624) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraffin-embedded Mouse spleen tissue using Mouse Mki67 Rabbit pAb (AAA28624) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Mouse Mki67 Rabbit pAb (AAA28624) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Mouse Mki67 Rabbit pAb (AAA28624) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Mouse Mki67 Rabbit pAb (AAA28624) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Mouse Mki67 Rabbit pAb (AAA28624) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Mouse Mki67 Rabbit pAb (AAA28624) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

Related Product Information for anti-Mki67 antibody

Predicted to enable protein C-terminus binding activity. Involved in regulation of chromatin organization. Acts upstream of or within cholangiocyte proliferation; hepatocyte proliferation; and meiotic cell cycle. Located in chromosome; cytoplasm; and nucleolus. Is expressed in several structures, including alimentary system; brain; genitourinary system; sensory organ; and skin. Human ortholog(s) of this gene implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).

Product Categories/Family for anti-Mki67 antibody

Neuroscience; Cell Type Marker; Neuron marker; Soma marker; Cell Cycle; Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 169234624
• NCBI GeneID: 17345
• NCBI Accession #: NP_001074586.2
• NCBI GenBank Nucleotide #: NM_001081117.2
• UniProt Accession #: E9PVX6; Q61769; Q7TSF6
• Molecular Weight: Calculated MW: 350kDa
• UniProt Protein Name: Proliferation marker protein Ki-67
• UniProt Gene Name: Mki67
• UniProt Synonym Gene Names: Antigen KI-67 homologCurated; Antigen Ki67 homologCurated