WB (Western Blot)
(Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody AAA211264 (lot AAA211264-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human MKRN1 Polyclonal Antibody | anti-MKRN1 antibody
Rabbit anti-MKRN1 Antibody, Affinity Purified
Gene Names
MKRN1; RNF61
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MKRN1, Antibody; Rabbit anti-MKRN1 Antibody, Affinity Purified; E3 ubiquitin-protein ligase makorin-1; RNF61; RING finger protein 61; RING-type E3 ubiquitin transferase makorin-1; makorin ring finger protein 1; anti-MKRN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MKRN1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 432 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody AAA211264 (lot AAA211264-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody AAA211264 (lot AAA211264-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MKRN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MKRN1 antibody AAA211264 (lot AAA211264-2) used for IP at 6 ug per reaction. MKRN1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211264-1). For blotting immunoprecipitated MKRN1, AAA211264 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human MKRN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MKRN1 antibody AAA211264 (lot AAA211264-2) used for IP at 6 ug per reaction. MKRN1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211264-1). For blotting immunoprecipitated MKRN1, AAA211264 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-MKRN1 antibody
MKRN1 was identified as a repressor of c-Jun, androgen receptor, and retinoic acid receptor transcriptional activity. MKRN1 is a RING zinc finger protein that has been demonstrated to function as an E3 ubiquitin ligase. The repressive activity of MKRN1 is not dependent on its ubiquitin ligase activity. MKRN1 also appears to function as a transactivator of RNA polymerase II dependent transcription.
Product Categories/Family for anti-MKRN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,224 Da
NCBI Official Full Name
makorin ring finger protein 1
NCBI Official Synonym Full Names
makorin ring finger protein 1
NCBI Official Symbol
MKRN1
NCBI Official Synonym Symbols
RNF61
NCBI Protein Information
E3 ubiquitin-protein ligase makorin-1
UniProt Protein Name
E3 ubiquitin-protein ligase makorin-1
UniProt Gene Name
MKRN1
UniProt Synonym Gene Names
RNF61
UniProt Entry Name
MKRN1_HUMAN
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Product Notes
The MKRN1 mkrn1 (Catalog #AAA211264) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MKRN1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MKRN1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MKRN1 mkrn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MKRN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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