IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit MLK1 Polyclonal Antibody | anti-MAP3K9 antibody
MLK1 Antibody (C-term)
Gene Names
MAP3K9; MLK1; MEKK9; PRKE1
Reactivity
Human, mouse, rat
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MLK1, Antibody; MLK1 Antibody (C-term); Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1; MAP3K9; MLK1; PRKE1; anti-MAP3K9 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1070-1104 amino acids from the C-terminal region of human MLK1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1070-1104
Sequence Length
1,118
Applicable Applications for anti-MAP3K9 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-MLK1 Pab is used in Western blot to detect MLK1 in A375 cell lysate (lane 1) and rat testis tissue lysate (lane 2).)
WB (Western Blot)
(The anti-MLK1 Pab is used in Western blot to detect MLK1 in A375 cell lysate (lane 1) and rat testis tissue lysate (lane 2).)
Related Product Information for anti-MAP3K9 antibody
MLK1 is a MLK(MAP3K) type protein kinase. The catalytic domain of mixed-lineage kinases (MLKs) kinases have amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition to thee unusual nature of the kinase catalytic domains, MLK1 and MLK2 contain 2 leu/ile-zipper motifs and a basic sequence near their C-termini. MLK1 is a member of the neuronal apoptotic JNK/c-Jun pathway acting between Rac1/Cdc42 and MKK4 and -7 in death signaling. MLK1 expression has been documented in human epithelial tumor cell lines of colonic, breast and esophageal origin.
Product Categories/Family for anti-MAP3K9 antibody
References
Durkin, J.T., et al., Biochemistry 43(51):16348-16355 (2004).
Gallo, K.A., et al., J. Biol. Chem. 269(21):15092-15100 (1994).
Dorow, D.S., et al., Eur. J. Biochem. 213(2):701-710 (1993).
Gallo, K.A., et al., J. Biol. Chem. 269(21):15092-15100 (1994).
Dorow, D.S., et al., Eur. J. Biochem. 213(2):701-710 (1993).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121895
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 9 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 9
NCBI Official Symbol
MAP3K9
NCBI Official Synonym Symbols
MLK1; MEKK9; PRKE1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 9
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 9
UniProt Gene Name
MAP3K9
UniProt Synonym Gene Names
MLK1; PRKE1
UniProt Entry Name
M3K9_HUMAN
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Product Notes
The MAP3K9 map3k9 (Catalog #AAA289826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1070-1104. The MLK1 Antibody (C-term) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's MLK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAP3K9 map3k9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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