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IHC (Immunohistochemistry)
(At 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Rabbit MLK3 Polyclonal Antibody | anti-MLK3 antibody
Phospho-MLK3 (Thr277+Ser281) Antibody
Gene Names
MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
MLK3, Antibody; Phospho-MLK3 (Thr277+Ser281) Antibody; 2610017K16Rik; EC 2.7.11.25; M3K11_HUMAN; Map3k11; MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1; PTK1; RHOE; SH3 domain containing proline rich kinase; SPRK; Src-homology 3 domain-containing proline-rich kinase; anti-MLK3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-MLK3 (Thr277+Ser281) Antibody detects endogenous levels of MLK3 only when phosphorylated at Thr277+Ser281.
Purity/Purification
Peptide affinity purification
Form/Format
Supplied in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-MLK3 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human MLK3 around the phosphorylation site of Thr277+Ser281.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of MLK3 (Phospho-Thr277+Ser281) using K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of MLK3 (Phospho-Thr277+Ser281) using K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using MLK3 (Phospho-Thr277+Ser281) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using MLK3 (Phospho-Thr277+Ser281) Antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
92KD
NCBI Official Full Name
Mitogen-activated protein kinase kinase kinase 11
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 11
NCBI Official Symbol
MAP3K11
NCBI Official Synonym Symbols
MLK3; PTK1; SPRK; MLK-3; MEKK11
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 11
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 11
UniProt Gene Name
MAP3K11
UniProt Entry Name
M3K11_HUMAN
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Product Notes
The MLK3 map3k11 (Catalog #AAA329670) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MLK3 (Thr277+Ser281) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MLK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MLK3 map3k11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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