WB (Western Blot)
(Western blot analysis of extracts from ?MCF-7 cells, using MLN51/CASC3 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit MLN51/CASC3 Polyclonal Antibody | anti-MLN51/CASC3 antibody
MLN51/CASC3 Antibody
Gene Names
CASC3; BTZ; MLN51
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
MLN51/CASC3, Antibody; MLN51/CASC3 Antibody; Barentsz; Barentsz protein; Btz; Cancer susceptibility candidate 3; Cancer susceptibility candidate gene 3 protein; CASC3; CASC3_HUMAN; Metastatic lymph node 51; Metastatic lymph node gene 51 protein; Metastatic lymph node protein 51; MLN 51; MLN 51 protein; MLN51; Protein barentsz; Protein CASC3; Protein MLN 51; anti-MLN51/CASC3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
MLN51/CASC3 Antibody detects endogenous levels of MLN51/CASC3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
703
Applicable Applications for anti-MLN51/CASC3 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human MLN51/CASC3.
Tissue Specificity
Widely expressed. Overexpressed in breast cancers and metastasis, as well as in gastric cancers.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from ?MCF-7 cells, using MLN51/CASC3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from ?MCF-7 cells, using MLN51/CASC3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela, using MLN51/CASC3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela, using MLN51/CASC3 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-MLN51/CASC3 antibody
Description: Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer.
Post Translational Modifications: ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
Subunit Structure: Component of the mRNA splicing-dependent exon junction complex (EJC), which contains at least CASC3, EIF4A3, MAGOH, NXF1 and RBM8A/Y14 (PubMed:15166247, PubMed:16170325, PubMed:16314458, PubMed:16923391, PubMed:16931718, PubMed:19033377). Forms homooligomers (By similarity). Interacts with STAU in an RNA-dependent manner (By similarity).
Similarity: The coiled coil domain may be involved in oligomerization. Belongs to the CASC3 family.
Post Translational Modifications: ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
Subunit Structure: Component of the mRNA splicing-dependent exon junction complex (EJC), which contains at least CASC3, EIF4A3, MAGOH, NXF1 and RBM8A/Y14 (PubMed:15166247, PubMed:16170325, PubMed:16314458, PubMed:16923391, PubMed:16931718, PubMed:19033377). Forms homooligomers (By similarity). Interacts with STAU in an RNA-dependent manner (By similarity).
Similarity: The coiled coil domain may be involved in oligomerization. Belongs to the CASC3 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 120 kDa
Predicted: 77 kDa
Predicted: 77 kDa
NCBI Official Full Name
protein CASC3
NCBI Official Synonym Full Names
CASC3 exon junction complex subunit
NCBI Official Symbol
CASC3
NCBI Official Synonym Symbols
BTZ; MLN51
NCBI Protein Information
protein CASC3
UniProt Protein Name
Protein CASC3
UniProt Gene Name
CASC3
UniProt Synonym Gene Names
MLN51; MLN 51; Btz
UniProt Entry Name
CASC3_HUMAN
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Product Notes
The MLN51/CASC3 casc3 (Catalog #AAA328685) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLN51/CASC3 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's MLN51/CASC3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the MLN51/CASC3 casc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLN51/CASC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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