IHC (Immunohistochemisry)
(MMP13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit MMP13 Polyclonal Antibody | anti-MMP13 antibody
MMP13 Antibody (Center)
Gene Names
MMP13; CLG3; MANDP1; MMP-13
Reactivity
Human (Predicted Reactivity: Rabbit)
Applications
Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MMP13, Antibody; MMP13 Antibody (Center); Collagenase 3; 3424-; Matrix metalloproteinase-13; MMP-13; MMP13; anti-MMP13 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rabbit)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MMP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the Central region of human MMP13.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
253-282
Sequence Length
471
Applicable Applications for anti-MMP13 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(MMP13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(MMP13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP13 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP13 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(MMP13 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MMP13 antibody detected the MMP13 protein (arrow).)
WB (Western Blot)
(MMP13 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MMP13 antibody detected the MMP13 protein (arrow).)
Related Product Information for anti-MMP13 antibody
Proteins of the matrix metalloproteinase (MMP) family are
involved in the breakdown of extracellular matrix in normal
physiological processes, such as embryonic development,
reproduction, and tissue remodeling, as well as in disease
processes, such as arthritis and metastasis. Most MMP's are
secreted as inactive proproteins which are activated when cleaved
by extracellular proteinases. The protein encoded by this gene
cleaves type II collagen more efficiently than types I and III. It
may be involved in articular cartilage turnover and cartilage
pathophysiology associated with osteoarthritis. The gene is part of
a cluster of MMP genes which localize to chromosome 11q22.3.
involved in the breakdown of extracellular matrix in normal
physiological processes, such as embryonic development,
reproduction, and tissue remodeling, as well as in disease
processes, such as arthritis and metastasis. Most MMP's are
secreted as inactive proproteins which are activated when cleaved
by extracellular proteinases. The protein encoded by this gene
cleaves type II collagen more efficiently than types I and III. It
may be involved in articular cartilage turnover and cartilage
pathophysiology associated with osteoarthritis. The gene is part of
a cluster of MMP genes which localize to chromosome 11q22.3.
Product Categories/Family for anti-MMP13 antibody
References
Miranda, K.J., et al. J. Biol. Chem. 285(41):31517-31524(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010)
Yamada, T., et al. Anticancer Res. 30(7):2693-2699(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010)
Yamada, T., et al. Anticancer Res. 30(7):2693-2699(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53820
NCBI Official Full Name
collagenase 3 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 13
NCBI Official Symbol
MMP13
NCBI Official Synonym Symbols
CLG3; MANDP1; MMP-13
NCBI Protein Information
collagenase 3
UniProt Protein Name
Collagenase 3
UniProt Gene Name
MMP13
UniProt Synonym Gene Names
MMP-13
UniProt Entry Name
MMP13_HUMAN
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Product Notes
The MMP13 mmp13 (Catalog #AAA284224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 253-282. The MMP13 Antibody (Center) reacts with Human (Predicted Reactivity: Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's MMP13 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MMP13 mmp13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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