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product-image-AAA212241_WB13.jpg WB (Western Blot) (Detection of human MNT by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, LNCaP, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MNT antibody (AAA212241 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

Rabbit anti-Human MNT Polyclonal Antibody | anti-MNT antibody

Rabbit anti-MNT Antibody, Affinity Purified

Gene Names
MNT; ROX; MAD6; MXD6; bHLHd3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MNT, Antibody; Rabbit anti-MNT Antibody, Affinity Purified; ROX; class D basic helix-loop-helix protein 3; MAD6; MAX binding protein; max-binding protein MNT; Max-interacting protein; MNT, MAX dimerization protein; MXD6; myc antagonist MNT; Protein ROX; bHLHd3; Max-binding protein MNT; MAX network transcriptional repressor; anti-MNT antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MNT antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human MNT by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, LNCaP, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MNT antibody (AAA212241 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA212241_WB13.jpg WB (Western Blot) (Detection of human MNT by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, LNCaP, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MNT antibody (AAA212241 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation)

(Detection of human MNT by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MNT antibody (AAA212241 lot 2) used for IP at 6 ug per reaction. MNT was also immunoprecipitated by a previous lot of this antibody (AAA212241 lot 1) and another antibody against a different epitope of MNT . For blotting immunoprecipitated MNT, AAA212241 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

product-image-AAA212241_IP15.jpg IP (Immunoprecipitation) (Detection of human MNT by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MNT antibody (AAA212241 lot 2) used for IP at 6 ug per reaction. MNT was also immunoprecipitated by a previous lot of this antibody (AAA212241 lot 1) and another antibody against a different epitope of MNT . For blotting immunoprecipitated MNT, AAA212241 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-MNT antibody
Max-binding protein MNT binds DNA as a heterodimer with MAX and represses transcription. MNT binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3' [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99583].
Product Categories/Family for anti-MNT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,300 Da
NCBI Official Full Name
max-binding protein MNT
NCBI Official Synonym Full Names
MNT, MAX dimerization protein
NCBI Official Symbol
MNT
NCBI Official Synonym Symbols
ROX; MAD6; MXD6; bHLHd3
NCBI Protein Information
max-binding protein MNT; myc antagonist MNT; MAX binding protein; Max-interacting protein; class D basic helix-loop-helix protein 3
UniProt Protein Name
Max-binding protein MNT
UniProt Gene Name
MNT
UniProt Synonym Gene Names
BHLHD3; ROX; bHLHd3
UniProt Entry Name
MNT_HUMAN

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Product Notes

The MNT mnt (Catalog #AAA212241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MNT Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MNT can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MNT mnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MNT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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