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IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit MOB4A Polyclonal Antibody | anti-MOB1B antibody
MOB4A Antibody (C-term)
Gene Names
MOB1B; MATS2; MOB4A; MOBKL1A
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MOB4A, Antibody; MOB4A Antibody (C-term); MOB kinase activator 1B; Mob1 homolog 1A; Mob1A; Mob1B; Mps one binder kinase activator-like 1A; MOB1B; MOB4A; MOBKL1A; anti-MOB1B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
177-206
Sequence Length
221
Applicable Applications for anti-MOB1B antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody (C-term). NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).MOB4A immunoreactivity is localized to Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody (C-term). NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).MOB4A immunoreactivity is localized to Cytoplasm significantly.)
WB (Western Blot)
(MOB4A Antibody (C-term) western blot analysis in Daudi,K562 cell line lysates (35ug/lane).This demonstrates the MOB4A antibody detected the MOB4A protein (arrow).)
WB (Western Blot)
(MOB4A Antibody (C-term) western blot analysis in Daudi,K562 cell line lysates (35ug/lane).This demonstrates the MOB4A antibody detected the MOB4A protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term). AAA284240 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term). AAA284240 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-MOB1B antibody
MOB4A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob4A regulates NDR1 and NDR2 kinase activity, widely expressed nuclear serine-threonine kinases that have been implicated in cell proliferation and/or tumor progression.
Product Categories/Family for anti-MOB1B antibody
References
Bichsel,S.J.,J. Biol. Chem. 279 (34), 35228-35235 (2004) Devroe,E.,J. Biol. Chem. 279 (23), 24444-24451 (2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25091
NCBI Official Full Name
MOB kinase activator 1B isoform 1
NCBI Official Synonym Full Names
MOB kinase activator 1B
NCBI Official Symbol
MOB1B
NCBI Official Synonym Symbols
MATS2; MOB4A; MOBKL1A
NCBI Protein Information
MOB kinase activator 1B
UniProt Protein Name
MOB kinase activator 1B
UniProt Gene Name
MOB1B
UniProt Synonym Gene Names
MOB4A; MOBKL1A; Mob1A
UniProt Entry Name
MOB1B_HUMAN
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Product Notes
The MOB1B mob1b (Catalog #AAA284240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 177-206. The MOB4A Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's MOB4A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MOB1B mob1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOB4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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