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FCM/FACS (Flow Cytometry)
(MPP1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human MPP1 Polyclonal Antibody | anti-MPP1 antibody
MPP1 Antibody (Center)
Gene Names
MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MPP1, Antibody; MPP1 Antibody (Center); 55 kDa erythrocyte membrane protein; p55; Membrane protein; palmitoylated 1; MPP1; DXS552E; EMP55; anti-MPP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-327 amino acids from the Central region of human MPP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
301-327
Sequence Length
446
Applicable Applications for anti-MPP1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(MPP1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MPP1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(MPP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MPP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(MPP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MPP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of MPP1 (arrow) using rabbit polyclonal MPP1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPP1 gene.)
WB (Western Blot)
(Western blot analysis of MPP1 (arrow) using rabbit polyclonal MPP1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPP1 gene.)
WB (Western Blot)
(MPP1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MPP1 antibody detected the MPP1 protein (arrow).)
WB (Western Blot)
(MPP1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MPP1 antibody detected the MPP1 protein (arrow).)
Related Product Information for anti-MPP1 antibody
This gene encodes the prototype of the membrane-associated
guanylate kinase (MAGUK) family proteins. MAGUKs interact with the
cytoskeleton and regulate cell proliferation, signaling pathways,
and intercellular junctions. The encoded protein is an extensively
palmitoylated membrane phosphoprotein containing a PDZ domain, a
Src homology 3 (SH3) motif, and a guanylate kinase domain. This
gene product interacts with various cytoskeletal proteins and cell
junctional proteins in different tissue and cell types, and may be
involved in the regulation of cell shape, hair cell development,
neural patterning of the retina, and apico-basal polarity and tumor
suppression pathways in non-erythroid cells. Multiple transcript
variants encoding different isoforms have been found for this gene.
guanylate kinase (MAGUK) family proteins. MAGUKs interact with the
cytoskeleton and regulate cell proliferation, signaling pathways,
and intercellular junctions. The encoded protein is an extensively
palmitoylated membrane phosphoprotein containing a PDZ domain, a
Src homology 3 (SH3) motif, and a guanylate kinase domain. This
gene product interacts with various cytoskeletal proteins and cell
junctional proteins in different tissue and cell types, and may be
involved in the regulation of cell shape, hair cell development,
neural patterning of the retina, and apico-basal polarity and tumor
suppression pathways in non-erythroid cells. Multiple transcript
variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-MPP1 antibody
References
Seo, P.S., et al. Exp. Biol. Med. (Maywood) 234(3):255-262(2009)
Seo, P.S., et al. Biochim. Biophys. Acta 1793(2):281-289(2009)
Gosens, I., et al. Hum. Mol. Genet. 16(16):1993-2003(2007)
Kusunoki, H., et al. Proteins 64(3):804-807(2006)
Funke, L., et al. Annu. Rev. Biochem. 74, 219-245 (2005) :
Seo, P.S., et al. Biochim. Biophys. Acta 1793(2):281-289(2009)
Gosens, I., et al. Hum. Mol. Genet. 16(16):1993-2003(2007)
Kusunoki, H., et al. Proteins 64(3):804-807(2006)
Funke, L., et al. Annu. Rev. Biochem. 74, 219-245 (2005) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52296
NCBI Official Full Name
55 kDa erythrocyte membrane protein isoform 2
NCBI Official Synonym Full Names
membrane protein, palmitoylated 1, 55kDa
NCBI Official Symbol
MPP1
NCBI Official Synonym Symbols
MRG1; PEMP; AAG12; EMP55; DXS552E
NCBI Protein Information
55 kDa erythrocyte membrane protein
UniProt Protein Name
55 kDa erythrocyte membrane protein
UniProt Gene Name
MPP1
UniProt Synonym Gene Names
DXS552E; EMP55; p55
UniProt Entry Name
EM55_HUMAN
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Product Notes
The MPP1 mpp1 (Catalog #AAA285306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 301-327. The MPP1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MPP1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MPP1 mpp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MPP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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