IHC (Immunohiostchemistry)
(MRPS12 Antibody (Center K43) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPS12 Antibody (Center K43) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human MRPS12 Polyclonal Antibody | anti-MRPS12 antibody
MRPS12 Antibody (Center K43)
Gene Names
MRPS12; RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MRPS12, Antibody; MRPS12 Antibody (Center K43); 28S ribosomal protein S12; mitochondrial; MRP-S12; S12mt; MT-RPS12; MRPS12; RPMS12; RPSM12; anti-MRPS12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MRPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-59 amino acids from the Central region of human MRPS12.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
28-59
Sequence Length
138
Applicable Applications for anti-MRPS12 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(MRPS12 Antibody (Center K43) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPS12 Antibody (Center K43) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(MRPS12 Antibody (Center K43) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPS12 Antibody (Center K43) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(MRPS12 Antibody (Center K43) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MRPS12 antibody detected the MRPS12 protein (arrow).)
WB (Western Blot)
(MRPS12 Antibody (Center K43) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MRPS12 antibody detected the MRPS12 protein (arrow).)
Related Product Information for anti-MRPS12 antibody
Mammalian mitochondrial ribosomal proteins are encoded by
nuclear genes and help in protein synthesis within the
mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
small 28S subunit and a large 39S subunit. They have an estimated
75% protein to rRNA composition compared to prokaryotic ribosomes,
where this ratio is reversed. Another difference between mammalian
mitoribosomes and prokaryotic ribosomes is that the latter contain
a 5S rRNA. Among different species, the proteins comprising the
mitoribosome differ greatly in sequence, and sometimes in
biochemical properties, which prevents easy recognition by sequence
homology. This gene encodes a 28S subunit protein that belongs to
the ribosomal protein S12P family. The encoded protein is a key
component of the ribosomal small subunit and controls the decoding
fidelity and susceptibility to aminoglycoside antibiotics. The gene
for mitochondrial seryl-tRNA synthetase is located upstream and
adjacent to this gene, and both genes are possible candidates for
the autosomal dominant deafness gene (DFNA4). Splice variants that
differ in the 5' UTR have been found for this gene; all three
variants encode the same protein.
nuclear genes and help in protein synthesis within the
mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
small 28S subunit and a large 39S subunit. They have an estimated
75% protein to rRNA composition compared to prokaryotic ribosomes,
where this ratio is reversed. Another difference between mammalian
mitoribosomes and prokaryotic ribosomes is that the latter contain
a 5S rRNA. Among different species, the proteins comprising the
mitoribosome differ greatly in sequence, and sometimes in
biochemical properties, which prevents easy recognition by sequence
homology. This gene encodes a 28S subunit protein that belongs to
the ribosomal protein S12P family. The encoded protein is a key
component of the ribosomal small subunit and controls the decoding
fidelity and susceptibility to aminoglycoside antibiotics. The gene
for mitochondrial seryl-tRNA synthetase is located upstream and
adjacent to this gene, and both genes are possible candidates for
the autosomal dominant deafness gene (DFNA4). Splice variants that
differ in the 5' UTR have been found for this gene; all three
variants encode the same protein.
References
Zanotto, E., et al. Biochim. Biophys. Acta 1789(5):432-442(2009)
Russo, A., et al. Biochim. Biophys. Acta 1779(12):820-829(2008)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Cui, Y.P., et al. World J. Gastroenterol. 9(9):1892-1896(2003)
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Russo, A., et al. Biochim. Biophys. Acta 1779(12):820-829(2008)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Cui, Y.P., et al. World J. Gastroenterol. 9(9):1892-1896(2003)
Zhang, Z., et al. Genomics 81(5):468-480(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15173
NCBI Official Full Name
28S ribosomal protein S12, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S12
NCBI Official Symbol
MRPS12
NCBI Official Synonym Symbols
RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
NCBI Protein Information
28S ribosomal protein S12, mitochondrial
UniProt Protein Name
28S ribosomal protein S12, mitochondrial
UniProt Gene Name
MRPS12
UniProt Synonym Gene Names
RPMS12; RPSM12; MRP-S12; S12mt
UniProt Entry Name
RT12_HUMAN
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Product Notes
The MRPS12 mrps12 (Catalog #AAA288408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 28-59. The MRPS12 Antibody (Center K43) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRPS12 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MRPS12 mrps12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPS12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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