WB (Western Blot)
(Detection of human and mouse MSK2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MSK2 antibody AAA211995 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MSK2 was also immunoprecipitated by rabbit anti-MSK2 antibody BL9977, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
Rabbit anti-Human, Mouse MSK2 Polyclonal Antibody | anti-MSK2 antibody
Rabbit anti-MSK2 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MSK2, Antibody; Rabbit anti-MSK2 Antibody, Affinity Purified; MSK2; RSK-B; RSKB; S6K-alpha-4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase alpha-4; nuclear mitogen- and stress-activated protein kinase 2; mitogen- and stress-activated protein kinase 2; 90 kDa ribosomal protein S6 kinase 4; ribosomal protein kinase B; Ribosomal protein S6 kinase alpha-4; ribosomal protein S6 kinase A4; anti-MSK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-MSK2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 722 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse MSK2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MSK2 antibody AAA211995 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MSK2 was also immunoprecipitated by rabbit anti-MSK2 antibody BL9977, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
WB (Western Blot)
(Detection of human and mouse MSK2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MSK2 antibody AAA211995 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MSK2 was also immunoprecipitated by rabbit anti-MSK2 antibody BL9977, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human MSK2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MSK2 (Cat. No. AAA211995) used at a dilution of 1:200 (1ug/ml) Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MSK2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MSK2 (Cat. No. AAA211995) used at a dilution of 1:200 (1ug/ml) Detection: DAB)
Related Product Information for anti-MSK2 antibody
MSK1 is a serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). MSK1 Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. MSK1 also phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75582].
Product Categories/Family for anti-MSK2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The MSK2 rps6ka4 (Catalog #AAA211995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MSK2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MSK2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MSK2 rps6ka4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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