Rabbit anti-Human MT-CO3 Polyclonal Antibody | anti-MT-CO3 antibody

MT-CO3 Antibody (N-term)

Cat. Num. AAA289771

• Gene Names: MT-CO3; COIII; MTCO3; COX3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: MT-CO3, Antibody; MT-CO3 Antibody (N-term); Cytochrome c oxidase subunit 3; Cytochrome c oxidase polypeptide III; MT-CO3; COIII; COXIII; MTCO3; anti-MT-CO3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MT-CO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human MT-CO3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 48-77
• Sequence Length: 261

• Applicable Applications for anti-MT-CO3 antibody: ELISA, WB (Western Blot)
• Function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
• Cellular Location: Mitochondrion inner membrane; Multi-pass membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(MT-CO3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MT-CO3 antibody detected the MT-CO3 protein (arrow).)

Related Product Information for anti-MT-CO3 antibody

Subunits I, II and III form the functional core of the enzyme complex.

Product Categories/Family for anti-MT-CO3 antibody

Cancer; Cardiovascular; Cell biology; Metabolism; Signal transduction

References

Andrews, R.M., et al. Nat. Genet. 23 (2), 147 (1999) :
Anderson, S., et al. Nature 290(5806):457-465(1981)
Submitted (08-JUL-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA :
Kogelnik, A.M., et al. Submitted (24-AUG-2006) Mitomap.org, Center for Molecular and Mitochondrial Medicine and Genetics (MAMMAG) University of California, University of California, Irvine, Irvine, CA 92697-3940, USA :
Kogelnik, A.M., et al. Submitted (18-APR-1997) Center for Molecular Medicine, Emory University School of Medicine, 1462 Clifton Road, Suite 420, Atlanta, GA 30322, USA :

NCBI and Uniprot Product Information

• NCBI GI #: 251831113
• NCBI GeneID: 4514
• NCBI Accession #: YP_003024032.1
• NCBI GenBank Nucleotide #: NC_012920.1
• UniProt Accession #: P00414; Q14Y83
• Molecular Weight: 29951
• NCBI Official Full Name: cytochrome c oxidase subunit III (mitochondrion)
• NCBI Official Synonym Full Names: mitochondrially encoded cytochrome c oxidase III
• NCBI Official Symbol: MT-CO3
• NCBI Official Synonym Symbols: COIII; MTCO3; COX3
• NCBI Protein Information: cytochrome c oxidase III
• UniProt Protein Name: Cytochrome c oxidase subunit 3
• UniProt Gene Name: MT-CO3
• UniProt Synonym Gene Names: COIII; COXIII; MTCO3
• UniProt Entry Name: COX3_HUMAN