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IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (AAA28241) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit MTOR Polyclonal Antibody | anti-MTOR antibody
MTOR Polyclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
MTOR, Antibody; MTOR Polyclonal Antibody; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; anti-MTOR antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
2549
Applicable Applications for anti-MTOR antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2388-2487 of human mTOR (NP_004949.1).
Cross Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (AAA28241) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (AAA28241) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cellsusing mTOR Rabbit pAb (AAA28241) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cellsusing mTOR Rabbit pAb (AAA28241) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using MTOR antibody at dilution of 1:150 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using MTOR antibody at dilution of 1:150 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using mTORantibody (AAA28241) at dilution of 1:150 (40xlens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using mTORantibody (AAA28241) at dilution of 1:150 (40xlens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat ovary using mTOR antibody (AAA28241) at dilution of 1:150 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat ovary using mTOR antibody (AAA28241) at dilution of 1:150 (40x lens).)
WB (Western Blot)
(Western Blot analysis of extracts of various cell lines, using mTOR antibody (AAA28241) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western Blot analysis of extracts of various cell lines, using mTOR antibody (AAA28241) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Related Product Information for anti-MTOR antibody
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Product Categories/Family for anti-MTOR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 300kDa
Calculated MW: 288kDa
Calculated MW: 288kDa
NCBI Official Full Name
Serine/threonine-protein kinase mTOR
NCBI Official Synonym Full Names
mechanistic target of rapamycin
NCBI Official Symbol
MTOR
NCBI Official Synonym Symbols
SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
NCBI Protein Information
serine/threonine-protein kinase mTOR
UniProt Protein Name
Serine/threonine-protein kinase mTOR
UniProt Gene Name
MTOR
UniProt Synonym Gene Names
FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR
Similar Products
Product Notes
The MTOR mtor (Catalog #AAA28241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MTOR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the MTOR mtor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTOR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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