Rat Serine/threonine-protein kinase mTOR ELISA Kit | Mtor elisa kit

Rat Serine/threonine-protein kinase mTOR ELISA Kit

Cat. Num. AAA310129

• Gene Names: Mtor; Frap1; RAFT1
• Reactivity: Rat
• Synonyms: Serine/threonine-protein kinase mTOR; N/A; Rat Serine/threonine-protein kinase mTOR ELISA Kit; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of; Mtor elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural ratLeptin. No significant cross-reactivity or interference was observed.
• Samples: Serum, Plasma,Tissue Homogenates And Cell Culture Supernates And Other Biological Fluids
• Detection Range: 0.15ng/mL-10.0ng/mL.

• Preparation and Storage: Store at 4 degree C

Standard Curve (Sample)

Related Product Information for Mtor elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofratLeptinconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forLeptin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Leptin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Leptin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9845251
• NCBI GeneID: 56718
• NCBI Accession #: NP_063971.1
• NCBI GenBank Nucleotide #: NM_019906.1
• UniProt Accession #: P42346
• Molecular Weight: 288,794 Da
• NCBI Official Full Name: serine/threonine-protein kinase mTOR
• NCBI Official Synonym Full Names: mechanistic target of rapamycin
• NCBI Official Symbol: Mtor
• NCBI Official Synonym Symbols: Frap1; RAFT1
• NCBI Protein Information: serine/threonine-protein kinase mTOR
• UniProt Protein Name: Serine/threonine-protein kinase mTOR
• UniProt Gene Name: Mtor
• UniProt Synonym Gene Names: Frap1; Raft1; mTOR; RAPT1