Rabbit anti-Human MUC1 Polyclonal Antibody | anti-MUC1 antibody

Anti-MUC1 Antibody, Rabbit Polyclonal

Cat. Num. AAA255524

• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MUC1, Antibody; Anti-MUC1 Antibody, Rabbit Polyclonal; MUC1 Antibody, Rabbit PAb, Antigen Affinity Purified; mucin 1, cell surface associated; Anti-ADMCKD Antibody; Anti-ADMCKD1 Antibody; Anti-CA15-3 Antibody; Anti-CD227 Antibody; Anti-EMA Antibody; Anti-H23AG Antibody; Anti-KL-6 Antibody; Anti-MAM6 Antibody; Anti-MCD Antibody; Anti-MCKD Antibody; Anti-MCKD1 Antibody; Anti-MUC-1 Antibody; Anti-MUC-1/SEC Antibody; Anti-MUC-1/X Antibody; Anti-MUC1/ZD Antibody; Anti-Mucin 1 Antibody; Anti-PEM Antibody; Anti-PEMT Antibody; Anti-PUM Antibody; anti-MUC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MUC1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MUC1 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human MUC1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human MUC1 (rh MUC1; NP_001018016.1; aa1-167). MUC1 specific IgG was purified by Human MUC1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(MUC1-rFc(167 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell LysateLane B:0.5 mg MCF7 Whole Cell Lysate4 uL anti-MUC1-rFc(167 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MUC1-rFc(167 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 122 kDaObserved band size :122 kDa)

WB (Western Blot)

(Anti-MUC1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:122 kDa)

Related Product Information for anti-MUC1 antibody

Mucin 1, cell surface-associated (MUC1) or polymorphic epithelial mucin (PEM) is a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach, and pancreas. MUC-1/CC1/CD227 Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized, and recycled to the cell membrane. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. The alpha subunit has cell adhesive properties. MUC-1/CC1/CD227 Can act both as an adhesion and an anti-adhesion protein. This protein May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. MUC-1/CC1/CD227 participated in modulates signaling in ERK, SRC, and NF-kappa-B pathways. In the activated T-cells, MUC-1/CC1/CD227 influences directly or indirectly the Ras/MAPK pathway. MUC-1/CC1/CD227 Promotes tumor progression and regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Product Categories/Family for anti-MUC1 antibody

Cancer Drug Targets; EGFR Signaling Pathway

References

Brayman M, et al. (2004) MUC1: a multifunctional cell surface component of reproductive tissue epithelia. Reprod Biol Endocrinol 2: 4.
Schroeder J A, et al. (2001) Transgenic MUC1 interacts with epidermal growth factor receptor and correlates with mitogen-activated protein kinase activation in the mouse mammary gland. J Biol Chem. 276 (16): 13057-64.
Li Y, et al. (2001) The epidermal growth factor receptor regulates interaction of the human DF3/MUC1 carcinoma antigen with c-Src and beta-catenin. J Biol Chem. 276 (38): 35239-42.