Rabbit anti-Human, mouse MUSK Polyclonal Antibody | anti-MUSK antibody

MUSK Antibody

Cat. Num. AAA28697

• Gene Names: MUSK; CMS9
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Rabbit Polyclonal Antibody (PAB)
• Synonyms: MUSK, Antibody; MUSK Antibody; Muscle; skeletal receptor tyrosine-protein kinase; Muscle-specific tyrosine-protein kinase receptor; MuSK; Muscle-specific kinase receptor; MUSK; anti-MUSK antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This MUSK antibody is generated from rabbits immunized with human MUSK recombinant protein.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (PAB)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Length: 783

• Applicable Applications for anti-MUSK antibody: WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence)
• Application Notes: WB~~1:1000; FC~~1:10~50; IF~~1:10~50
• Function: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle.Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation (By similarity).
• Cellular Location: Postsynaptic cell membrane; Single-pass type I membrane protein. Note=Colocalizes with acetylcholine receptors (AChR) to the postsynaptic cell membrane of the neuromuscular junction
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(MUSK Antibody (AAA28697) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Western blot analysis of anti-MUSK Pab (AAA28697) in mouse kidney tissue lysates (35ug/lane). MUSK(arrow) was detected using the purified Pab.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of MUSK Antibody (AAA28697) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-MUSK antibody

MuSK activates signaling cascades responsible for many aspects of synapse formation, including the organization of the postsynaptic membrane, synapse-specific transcription, and presynaptic differentiation. MuSK also mediates agrin-induced aggregation of acetylcholine receptors at the mature vertebrate neuromuscular junction and also during synapse formation. It has been shown that a majority of AChR Ab-seronegative myasthenia gravis patients possess serum autoantibodies against MuSK.

Product Categories/Family for anti-MUSK antibody

Signal Transduction

References

Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

NCBI and Uniprot Product Information

• NCBI GI #: 261878455
• NCBI GeneID: 4593
• NCBI Accession #: NP_001159752.1; NP_001159753.1; NP_005583.1
• NCBI GenBank Nucleotide #: NM_001166280.1
• UniProt Accession #: O15146; Q32MJ8; Q32MJ9; Q5VZW7; Q5VZW8
• Molecular Weight: 97056
• NCBI Official Full Name: muscle, skeletal receptor tyrosine-protein kinase isoform 2
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: MUSK
• NCBI Official Synonym Symbols: CMS9
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• UniProt Gene Name: MUSK
• UniProt Synonym Gene Names: MuSK; Muscle-specific kinase receptor
• UniProt Entry Name: MUSK_HUMAN

Frequently Asked Questions

What is MuSK and why is it important in neuromuscular research?

MuSK (Muscle-Specific Kinase) is a receptor tyrosine kinase essential for neuromuscular junction (NMJ) formation and maintenance. Researchers study MuSK to understand NMJ development, myasthenia gravis pathogenesis (MuSK-MG represents ~40% of seronegative cases), and mechanisms of neuromuscular transmission disorders affecting muscle function.

Can a MuSK antibody be used to detect MuSK expression at the neuromuscular junction?

Yes, MuSK antibodies specifically detect endogenous MuSK protein at the neuromuscular junction, where it localizes at the postsynaptic membrane. This enables researchers to visualize NMJ structure, assess MuSK expression levels, detect post-translational modifications, and study MuSK clustering and localization alterations in disease models.

Is MuSK antibody suitable for immunofluorescence or Western blot applications?

Yes, MuSK antibodies are validated for both immunofluorescence (enabling subcellular NMJ localization studies) and Western blotting (quantifying MuSK protein levels across tissues and experimental conditions). These complementary applications allow a comprehensive assessment of MuSK expression and post-translational modification status.

How does MuSK dysfunction relate to myasthenia gravis or muscle disorders?

MuSK autoantibodies (found in MuSK-MG patients) inhibit MuSK phosphorylation and downstream signaling, disrupting acetylcholine receptor (AChR) clustering and synaptic differentiation at the NMJ. This results in impaired neuromuscular transmission, characteristic muscle weakness, fatigue, and, in severe cases, respiratory insufficiency requiring ventilator support.

Does MuSK expression change during muscle development or injury?

Yes, MuSK expression is dynamically regulated during development, being highly expressed during NMJ formation. MuSK levels remain elevated at mature NMJs for junction maintenance. Following muscle injury or denervation, MuSK expression increases as part of neuromuscular repair responses and regeneration processes involving NMJ remodeling.

Can this antibody be used for both human and animal tissue analysis?

Yes, MuSK antibodies show cross-species reactivity with human, rodent, and other mammalian species. This enables researchers to study human patient samples (biopsies, autopsies), translate findings between animal models and clinical contexts, and assess MuSK pathology across diverse experimental and diagnostic applications.