IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MUTYH Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human, mouse MUTYH Polyclonal Antibody | anti-MUTYH antibody
MUTYH Antibody (Center)
Gene Names
MUTYH; MYH
Reactivity
Human, mouse
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MUTYH, Antibody; MUTYH Antibody (Center); A/G-specific adenine DNA glycosylase; 322-; MutY homolog; hMYH; MUTYH; MYH; anti-MUTYH antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MUTYH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-367 amino acids from the Central region of human MUTYH.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
341-367
Sequence Length
521
Applicable Applications for anti-MUTYH antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MUTYH Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MUTYH Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(MUTYH Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MUTYH Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of MUTYH Antibody (Center) in mouse lung tissue lysates (35ug/lane). MUTYH (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of MUTYH Antibody (Center) in mouse lung tissue lysates (35ug/lane). MUTYH (arrow) was detected using the purified Pab.)
Related Product Information for anti-MUTYH antibody
MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches. MYH is involved in oxidative DNA damage repair. It initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. It possesses both adenine and 2-OH-A DNA glycosylase activities.
References
Kim C.J., et.al., Oncogene 23:6820-6822(2004).
Isidro G., et.al., Hum. Mutat. 24:353-354(2004).
Sieber O.M., et.al., N. Engl. J. Med. 348:791-799(2003).
Isidro G., et.al., Hum. Mutat. 24:353-354(2004).
Sieber O.M., et.al., N. Engl. J. Med. 348:791-799(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60069
NCBI Official Full Name
A/G-specific adenine DNA glycosylase isoform 2
NCBI Official Synonym Full Names
mutY DNA glycosylase
NCBI Official Symbol
MUTYH
NCBI Official Synonym Symbols
MYH
NCBI Protein Information
A/G-specific adenine DNA glycosylase
UniProt Protein Name
A/G-specific adenine DNA glycosylase
UniProt Gene Name
MUTYH
UniProt Synonym Gene Names
MYH; hMYH
UniProt Entry Name
MUTYH_HUMAN
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Product Notes
The MUTYH mutyh (Catalog #AAA285272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 341-367. The MUTYH Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MUTYH can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MUTYH mutyh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUTYH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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