WB (Western Blot)
(Western blot analysis of extracts from rat brain, using MYOCD Antibody.)
Rabbit MYOCD Polyclonal Antibody | anti-MYOCD antibody
MYOCD Antibody
Gene Names
MYOCD; MYCD
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MYOCD, Antibody; MYOCD Antibody; MYCD; MYCD_HUMAN; Myocardin; Myocd; anti-MYOCD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MYOCD antibody detects endogenous levels of total MYOCD
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
986
Applicable Applications for anti-MYOCD antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
Predicted Cross Reactivity
Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Bovine (89%), Horse (89%), Rabbit (100%), Dog (89%), Chicken (100%), Xenopus (88%)
Conjugation
Unconjugated
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using MYOCD Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using MYOCD Antibody.)
WB (Western Blot)
(Western blot analysis of MYOCD expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of MYOCD expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-MYOCD antibody
Description: Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
Function: Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).
Subunit Structure: Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts with MLLT7/FOXO4. Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).
Post-translational Modifications: Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.
Similarity: The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).
Function: Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).
Subunit Structure: Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts with MLLT7/FOXO4. Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).
Post-translational Modifications: Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.
Similarity: The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 100 kDa
Predicted: 102 kDa
Predicted: 102 kDa
NCBI Official Full Name
myocardin isoform 1
NCBI Official Synonym Full Names
myocardin
NCBI Official Symbol
MYOCD
NCBI Official Synonym Symbols
MYCD
NCBI Protein Information
myocardin
UniProt Protein Name
Myocardin
UniProt Gene Name
MYOCD
UniProt Synonym Gene Names
MYCD
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Product Notes
The MYOCD myocd (Catalog #AAA323424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYOCD Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYOCD can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MYOCD myocd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYOCD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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